{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mirabelli C"],"funding":["HHS | National Institutes of Health","University of Michigan","DELTAS Africa grant","European Union","NIDDK NIH HHS","NIAID NIH HHS","New Zealand Ministry for Business Innovation and Employment grant","HHS | NIH | National Cancer Institute","NCI NIH HHS","National Institutes of Health","UM | Medical School, University of Michigan","NIH HHS"],"pagination":["e0017522"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9040803"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(2)"],"pubmed_abstract":["Human norovirus (HNoV) is a global health and socioeconomic burden, estimated to infect every individual at least five times during their lifetime. The underlying mechanism for the potential lack of long-term immune protection from HNoV infections is not understood and prompted us to investigate HNoV susceptibility of primary human B cells and its functional impact. Primary B cells isolated from whole blood were infected with HNoV-positive stool samples and harvested at 3 days postinfection (dpi) to assess the viral RNA yield by reverse transcriptase quantitative PCR (RT-qPCR). A 3- to 18-fold increase in the HNoV RNA yield was observed in 50 to 60% of donors. Infection was further confirmed in B cells derived from splenic and lymph node biopsy specimens. Next, we characterized infection o"],"journal":["mBio"],"pubmed_title":["Human Norovirus Triggers Primary B Cell Immune Activation <i>In Vitro</i>."],"pmcid":["PMC9040803"],"funding_grant_id":["DK097153","MICHR training grant (LT1)","R01 CA242003","UOOX1904","Kirschstein-NRSA F32 fellowship","R21 AI142032","R37CA237421","Charles Woodson Clinical Research Fund","R21 AI130328","Pediatric Brain Tumor Initiative","R21AI130328","R37 CA237421","Marie-Slodowska Curie global fellowship","R01AI123144","R01 AI141478","U24 DK097153","R01 AI123144","DEL-15-007: Awandare"],"pubmed_authors":["Wobus CE","Ward VK","Jones MK","Shan M","Turula H","Mirabelli C","Grigorova I","Trevino JG","Owusu I","Abuaita B","Karst SM","Kolawole AO","Lyssiotis CA","Young VL","Lundy SK"],"additional_accession":[]},"is_claimable":false,"name":"Human Norovirus Triggers Primary B Cell Immune Activation <i>In Vitro</i>.","description":"Human norovirus (HNoV) is a global health and socioeconomic burden, estimated to infect every individual at least five times during their lifetime. The underlying mechanism for the potential lack of long-term immune protection from HNoV infections is not understood and prompted us to investigate HNoV susceptibility of primary human B cells and its functional impact. Primary B cells isolated from whole blood were infected with HNoV-positive stool samples and harvested at 3 days postinfection (dpi) to assess the viral RNA yield by reverse transcriptase quantitative PCR (RT-qPCR). A 3- to 18-fold increase in the HNoV RNA yield was observed in 50 to 60% of donors. Infection was further confirmed in B cells derived from splenic and lymph node biopsy specimens. Next, we characterized infection o","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-06-10T03:19:08.205Z","creation":"2024-11-06T10:20:48.464Z"},"accession":"S-EPMC9040803","cross_references":{"pubmed":["35404121"],"doi":["10.1128/mbio.00175-22"]}}