{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Danziger O"],"funding":["NIAID NIH HHS","National Institutes of Health","NIH HHS","Icahn School of Medicine at Mount Sinai"],"pagination":["e1010464"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9041830"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(4)"],"pubmed_abstract":["Interferons establish an antiviral state through the induction of hundreds of interferon-stimulated genes (ISGs). The mechanisms and viral specificities for most ISGs remain incompletely understood. To enable high-throughput interrogation of ISG antiviral functions in pooled genetic screens while mitigating potentially confounding effects of endogenous interferon and antiproliferative/proapoptotic ISG activities, we adapted a CRISPR-activation (CRISPRa) system for inducible ISG expression in isogenic cell lines with and without the capacity to respond to interferons. We used this platform to screen for ISGs that restrict SARS-CoV-2. Results included ISGs previously described to restrict SARS-CoV-2 and novel candidate antiviral factors. We validated a subset of these by complementary CRISPR"],"journal":["PLoS pathogens"],"pubmed_title":["Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor."],"pmcid":["PMC9041830"],"funding_grant_id":["AI151029","S10OD026880","S10 OD030463","AI150748","S10 OD026880","S10OD018522","R01 AI151029","S10 OD018522","U01 AI150748"],"pubmed_authors":["Danziger O","Rosen MR","Rosenberg BR","Patel RS","DeGrace EJ"],"additional_accession":[]},"is_claimable":false,"name":"Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor.","description":"Interferons establish an antiviral state through the induction of hundreds of interferon-stimulated genes (ISGs). The mechanisms and viral specificities for most ISGs remain incompletely understood. To enable high-throughput interrogation of ISG antiviral functions in pooled genetic screens while mitigating potentially confounding effects of endogenous interferon and antiproliferative/proapoptotic ISG activities, we adapted a CRISPR-activation (CRISPRa) system for inducible ISG expression in isogenic cell lines with and without the capacity to respond to interferons. We used this platform to screen for ISGs that restrict SARS-CoV-2. Results included ISGs previously described to restrict SARS-CoV-2 and novel candidate antiviral factors. We validated a subset of these by complementary CRISPR","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-04-08T18:05:44.23Z","creation":"2025-04-06T21:51:35.244Z"},"accession":"S-EPMC9041830","cross_references":{"pubmed":["35421191"],"doi":["10.1371/journal.ppat.1010464"]}}