{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wan L"],"funding":["NIBIB NIH HHS","RRD VA","NINDS NIH HHS","National Institutes of Health","NIAMS NIH HHS","CSRD VA"],"pagination":["e4664"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9042587"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(5)"],"pubmed_abstract":["The objective of the current study was to investigate the feasibility of quantitative 3D ultrashort echo time (UTE)-based biomarkers in detecting proteoglycan (PG) loss and collagen degradation in human cartilage. A total of 104 cartilage samples were harvested for a trypsin digestion study (n = 44), and a sequential trypsin and collagenase digestion study (n = 60), respectively. Forty-four cartilage samples were randomly divided into a trypsin digestion group (tryp group) and a control group (phosphate-buffered saline [PBS] group) (n = 22 for each group) for the trypsin digestion experiment. The remaining 60 cartilage samples were divided equally into four groups (n = 15 for each group) for sequential trypsin and collagenase digestion, including PBS + Tris (incubated in PBS, then Tris buf"],"journal":["NMR in biomedicine"],"pubmed_title":["Evaluation of enzymatic proteoglycan loss and collagen degradation in human articular cartilage using ultrashort echo time-based biomarkers: A feasibility study."],"pmcid":["PMC9042587"],"funding_grant_id":["I01 CX001388","T32EB005970","R01AR075825","R01AR062581","R01 AR075825","R01NS092650","R01 NS092650","R21 AR075851","T32 EB005970","R01 AR062581","I01 RX002604","R21AR075851"],"pubmed_authors":["Ma YJ","Chang EY","Wan L","Wong JH","Searleman AC","Du J","Tang G","Cheng X","Meyer RS"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of enzymatic proteoglycan loss and collagen degradation in human articular cartilage using ultrashort echo time-based biomarkers: A feasibility study.","description":"The objective of the current study was to investigate the feasibility of quantitative 3D ultrashort echo time (UTE)-based biomarkers in detecting proteoglycan (PG) loss and collagen degradation in human cartilage. A total of 104 cartilage samples were harvested for a trypsin digestion study (n = 44), and a sequential trypsin and collagenase digestion study (n = 60), respectively. Forty-four cartilage samples were randomly divided into a trypsin digestion group (tryp group) and a control group (phosphate-buffered saline [PBS] group) (n = 22 for each group) for the trypsin digestion experiment. The remaining 60 cartilage samples were divided equally into four groups (n = 15 for each group) for sequential trypsin and collagenase digestion, including PBS + Tris (incubated in PBS, then Tris buf","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-04T14:46:11.371Z","creation":"2025-04-04T14:46:11.371Z"},"accession":"S-EPMC9042587","cross_references":{"pubmed":["34904305"],"doi":["10.1002/nbm.4664"]}}