biostudies-literatureWan LNIBIB NIH HHSRRD VANINDS NIH HHSNational Institutes of HealthNIAMS NIH HHSCSRD VAe4664https://www.ebi.ac.uk/biostudies/studies/S-EPMC9042587biostudies-literatureUnknown35(5)The objective of the current study was to investigate the feasibility of quantitative 3D ultrashort echo time (UTE)-based biomarkers in detecting proteoglycan (PG) loss and collagen degradation in human cartilage. A total of 104 cartilage samples were harvested for a trypsin digestion study (n = 44), and a sequential trypsin and collagenase digestion study (n = 60), respectively. Forty-four cartilage samples were randomly divided into a trypsin digestion group (tryp group) and a control group (phosphate-buffered saline [PBS] group) (n = 22 for each group) for the trypsin digestion experiment. The remaining 60 cartilage samples were divided equally into four groups (n = 15 for each group) for sequential trypsin and collagenase digestion, including PBS + Tris (incubated in PBS, then Tris bufNMR in biomedicineEvaluation of enzymatic proteoglycan loss and collagen degradation in human articular cartilage using ultrashort echo time-based biomarkers: A feasibility study.PMC9042587I01 CX001388T32EB005970R01AR075825R01AR062581R01 AR075825R01NS092650R01 NS092650R21 AR075851T32 EB005970R01 AR062581I01 RX002604R21AR075851Ma YJChang EYWan LWong JHSearleman ACDu JTang GCheng XMeyer RSfalseEvaluation of enzymatic proteoglycan loss and collagen degradation in human articular cartilage using ultrashort echo time-based biomarkers: A feasibility study.The objective of the current study was to investigate the feasibility of quantitative 3D ultrashort echo time (UTE)-based biomarkers in detecting proteoglycan (PG) loss and collagen degradation in human cartilage. A total of 104 cartilage samples were harvested for a trypsin digestion study (n = 44), and a sequential trypsin and collagenase digestion study (n = 60), respectively. Forty-four cartilage samples were randomly divided into a trypsin digestion group (tryp group) and a control group (phosphate-buffered saline [PBS] group) (n = 22 for each group) for the trypsin digestion experiment. The remaining 60 cartilage samples were divided equally into four groups (n = 15 for each group) for sequential trypsin and collagenase digestion, including PBS + Tris (incubated in PBS, then Tris buf2022-01-01T00:00:00Z2022 May2025-04-04T14:46:11.371Z2025-04-04T14:46:11.371ZS-EPMC90425873490430510.1002/nbm.4664