{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4(2)"],"submitter":["Oeyen AL"],"funding":["Heinrich-Heine-Universität Düsseldorf","Universität Duisburg-Essen"],"pubmed_abstract":["<h4>Purpose</h4>The purposes of this in vitro study were to investigate whether the addition of dexamethasone can compensate for any cytotoxic effects of the amide-type local anesthetics (LA) bupivacaine and ropivacaine and whether morphine and morphine-6-glucuronide (M6G) may be a safe alternative for peritendinous application.<h4>Methods</h4>Biopsies of human biceps tendons (<i>n</i> = 6) were dissected and cultivated. Cells were characterized by the expression for tenocyte markers, collagen I, biglycan, tenascin C, scleraxis, and RUNX via reverse transcriptase-polymerase chain reaction and immunohistochemistry. Tenocytes were incubated with bupivacaine, ropivacaine, morphine, M6G, or a saline control with and without addition of dexamethasone for 15, 60, or 240 min. Cell viability was d"],"journal":["Arthroscopy, sports medicine, and rehabilitation"],"pagination":["e459-e469"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9042774"],"repository":["biostudies-literature"],"pubmed_title":["Dexamethasone Does not Compensate for Local Anesthetic Cytotoxic Effects on Tenocytes: Morphine or Morphine Plus Dexamethasone May Be a Safe Alternative."],"pmcid":["PMC9042774"],"pubmed_authors":["Oeyen AL","Krauspe R","Herten M","Ickert I","Vogl M","Kircher J","Osada N","Bittersohl B"],"additional_accession":[]},"is_claimable":false,"name":"Dexamethasone Does not Compensate for Local Anesthetic Cytotoxic Effects on Tenocytes: Morphine or Morphine Plus Dexamethasone May Be a Safe Alternative.","description":"<h4>Purpose</h4>The purposes of this in vitro study were to investigate whether the addition of dexamethasone can compensate for any cytotoxic effects of the amide-type local anesthetics (LA) bupivacaine and ropivacaine and whether morphine and morphine-6-glucuronide (M6G) may be a safe alternative for peritendinous application.<h4>Methods</h4>Biopsies of human biceps tendons (<i>n</i> = 6) were dissected and cultivated. Cells were characterized by the expression for tenocyte markers, collagen I, biglycan, tenascin C, scleraxis, and RUNX via reverse transcriptase-polymerase chain reaction and immunohistochemistry. Tenocytes were incubated with bupivacaine, ropivacaine, morphine, M6G, or a saline control with and without addition of dexamethasone for 15, 60, or 240 min. Cell viability was d","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-05-29T21:53:50.208Z","creation":"2024-11-09T04:35:54.568Z"},"accession":"S-EPMC9042774","cross_references":{"pubmed":["35494256"],"doi":["10.1016/j.asmr.2021.11.004"]}}