biostudies-literatureUnknown4(2)Oeyen ALHeinrich-Heine-Universität DüsseldorfUniversität Duisburg-Essen<h4>Purpose</h4>The purposes of this in vitro study were to investigate whether the addition of dexamethasone can compensate for any cytotoxic effects of the amide-type local anesthetics (LA) bupivacaine and ropivacaine and whether morphine and morphine-6-glucuronide (M6G) may be a safe alternative for peritendinous application.<h4>Methods</h4>Biopsies of human biceps tendons (<i>n</i> = 6) were dissected and cultivated. Cells were characterized by the expression for tenocyte markers, collagen I, biglycan, tenascin C, scleraxis, and RUNX via reverse transcriptase-polymerase chain reaction and immunohistochemistry. Tenocytes were incubated with bupivacaine, ropivacaine, morphine, M6G, or a saline control with and without addition of dexamethasone for 15, 60, or 240 min. Cell viability was dArthroscopy, sports medicine, and rehabilitatione459-e469https://www.ebi.ac.uk/biostudies/studies/S-EPMC9042774biostudies-literatureDexamethasone Does not Compensate for Local Anesthetic Cytotoxic Effects on Tenocytes: Morphine or Morphine Plus Dexamethasone May Be a Safe Alternative.PMC9042774Oeyen ALKrauspe RHerten MIckert IVogl MKircher JOsada NBittersohl BfalseDexamethasone Does not Compensate for Local Anesthetic Cytotoxic Effects on Tenocytes: Morphine or Morphine Plus Dexamethasone May Be a Safe Alternative.<h4>Purpose</h4>The purposes of this in vitro study were to investigate whether the addition of dexamethasone can compensate for any cytotoxic effects of the amide-type local anesthetics (LA) bupivacaine and ropivacaine and whether morphine and morphine-6-glucuronide (M6G) may be a safe alternative for peritendinous application.<h4>Methods</h4>Biopsies of human biceps tendons (<i>n</i> = 6) were dissected and cultivated. Cells were characterized by the expression for tenocyte markers, collagen I, biglycan, tenascin C, scleraxis, and RUNX via reverse transcriptase-polymerase chain reaction and immunohistochemistry. Tenocytes were incubated with bupivacaine, ropivacaine, morphine, M6G, or a saline control with and without addition of dexamethasone for 15, 60, or 240 min. Cell viability was d2022-01-01T00:00:00Z2022 Apr2025-05-29T21:53:50.208Z2024-11-09T04:35:54.568ZS-EPMC90427743549425610.1016/j.asmr.2021.11.004