{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(4)"],"submitter":["Lv W"],"pubmed_abstract":["<h4>Background</h4>Extrachromosomal circular deoxyribonucleic acid (eccDNA) is evolving as a valuable biomarker, while little is known about its presence in urine.<h4>Methods</h4>Here, we report the discovery and analysis of urinary cell-free eccDNAs (ucf-eccDNAs) in healthy controls and patients with advanced chronic kidney disease (CKD) by Circle-Seq.<h4>Results</h4>Millions of unique ucf-eccDNAs were identified and comprehensively characterised. The ucf-eccDNAs are GC-rich. Most ucf-eccDNAs are less than 1000 bp and are enriched in four pronounced peaks at 207, 358, 553 and 732 bp. Analysis of the genomic distribution of ucf-eccDNAs shows that eccDNAs are found on all chromosomes but enriched on chromosomes 17, 19 and 20 with a high density of protein-coding genes, CpG islands, short in"],"journal":["Clinical and translational medicine"],"pagination":["e817"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9042798"],"repository":["biostudies-literature"],"pubmed_title":["Circle-Seq reveals genomic and disease-specific hallmarks in urinary cell-free extrachromosomal circular DNAs."],"pmcid":["PMC9042798"],"pubmed_authors":["Han P","Lv W","Bolund L","Qu K","Liu X","Wang Q","Zhang C","Wang Z","Pan X","Yang H","Li H","He F","Jin X","Yin T","Xing X","Zeng Y","Zheng T","Regenberg B","Li Y","Zhang X","Henriksen RA","Feng W","Luo Y","Liu C","Lin L","Xu Z"],"additional_accession":[]},"is_claimable":false,"name":"Circle-Seq reveals genomic and disease-specific hallmarks in urinary cell-free extrachromosomal circular DNAs.","description":"<h4>Background</h4>Extrachromosomal circular deoxyribonucleic acid (eccDNA) is evolving as a valuable biomarker, while little is known about its presence in urine.<h4>Methods</h4>Here, we report the discovery and analysis of urinary cell-free eccDNAs (ucf-eccDNAs) in healthy controls and patients with advanced chronic kidney disease (CKD) by Circle-Seq.<h4>Results</h4>Millions of unique ucf-eccDNAs were identified and comprehensively characterised. The ucf-eccDNAs are GC-rich. Most ucf-eccDNAs are less than 1000 bp and are enriched in four pronounced peaks at 207, 358, 553 and 732 bp. Analysis of the genomic distribution of ucf-eccDNAs shows that eccDNAs are found on all chromosomes but enriched on chromosomes 17, 19 and 20 with a high density of protein-coding genes, CpG islands, short in","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-05T13:23:25.25Z","creation":"2025-04-05T13:23:25.25Z"},"accession":"S-EPMC9042798","cross_references":{"pubmed":["35474296"],"doi":["10.1002/ctm2.817"]}}