{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(58)"],"submitter":["Yin Z"],"pubmed_abstract":["Immunoglobulin G (IgG) antibodies are an important class of biotherapeutics that target various diseases, such as cancers, neurodegenerative disorders, and autoimmune diseases, yet rapid discrimination of IgG antibodies remains a great challenge due to heterogeneity, flexibility, and large size. Herein, we demonstrate a simplified multicharge-state collision-induced unfolding (CIU) method for rapid differentiation of four IgG isotypes that differ in terms of the numbers and patterns of disulfide bonds, bypassing tedious single charge-state selection in advance. The results presented herein reveal that gas-phase unfolding behaviors have a strong dependence on charge states outside IgG surfaces; therefore, multicharge-state CIU analysis of IgG subtypes could offer a great opportunity to gain"],"journal":["RSC advances"],"pagination":["36502-36510"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9043582"],"repository":["biostudies-literature"],"pubmed_title":["Rapid structural discrimination of IgG antibodies by multicharge-state collision-induced unfolding."],"pmcid":["PMC9043582"],"pubmed_authors":["Zhang W","Huang W","Yan S","Du M","Yin Z","Chen D","Wu X"],"additional_accession":[]},"is_claimable":false,"name":"Rapid structural discrimination of IgG antibodies by multicharge-state collision-induced unfolding.","description":"Immunoglobulin G (IgG) antibodies are an important class of biotherapeutics that target various diseases, such as cancers, neurodegenerative disorders, and autoimmune diseases, yet rapid discrimination of IgG antibodies remains a great challenge due to heterogeneity, flexibility, and large size. Herein, we demonstrate a simplified multicharge-state collision-induced unfolding (CIU) method for rapid differentiation of four IgG isotypes that differ in terms of the numbers and patterns of disulfide bonds, bypassing tedious single charge-state selection in advance. The results presented herein reveal that gas-phase unfolding behaviors have a strong dependence on charge states outside IgG surfaces; therefore, multicharge-state CIU analysis of IgG subtypes could offer a great opportunity to gain","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-04-04T08:36:36.067Z","creation":"2025-02-18T23:21:11.866Z"},"accession":"S-EPMC9043582","cross_references":{"pubmed":["35494361"],"doi":["10.1039/d1ra06486j"]}}