biostudies-literatureUnknown11(58)Yin ZImmunoglobulin G (IgG) antibodies are an important class of biotherapeutics that target various diseases, such as cancers, neurodegenerative disorders, and autoimmune diseases, yet rapid discrimination of IgG antibodies remains a great challenge due to heterogeneity, flexibility, and large size. Herein, we demonstrate a simplified multicharge-state collision-induced unfolding (CIU) method for rapid differentiation of four IgG isotypes that differ in terms of the numbers and patterns of disulfide bonds, bypassing tedious single charge-state selection in advance. The results presented herein reveal that gas-phase unfolding behaviors have a strong dependence on charge states outside IgG surfaces; therefore, multicharge-state CIU analysis of IgG subtypes could offer a great opportunity to gainRSC advances36502-36510https://www.ebi.ac.uk/biostudies/studies/S-EPMC9043582biostudies-literatureRapid structural discrimination of IgG antibodies by multicharge-state collision-induced unfolding.PMC9043582Zhang WHuang WYan SDu MYin ZChen DWu XfalseRapid structural discrimination of IgG antibodies by multicharge-state collision-induced unfolding.Immunoglobulin G (IgG) antibodies are an important class of biotherapeutics that target various diseases, such as cancers, neurodegenerative disorders, and autoimmune diseases, yet rapid discrimination of IgG antibodies remains a great challenge due to heterogeneity, flexibility, and large size. Herein, we demonstrate a simplified multicharge-state collision-induced unfolding (CIU) method for rapid differentiation of four IgG isotypes that differ in terms of the numbers and patterns of disulfide bonds, bypassing tedious single charge-state selection in advance. The results presented herein reveal that gas-phase unfolding behaviors have a strong dependence on charge states outside IgG surfaces; therefore, multicharge-state CIU analysis of IgG subtypes could offer a great opportunity to gain2021-01-01T00:00:00Z2021 Nov2025-04-04T08:36:36.067Z2025-02-18T23:21:11.866ZS-EPMC90435823549436110.1039/d1ra06486j