{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(59)"],"submitter":["Kobayashi J"],"pubmed_abstract":["A heparin-modified poly(<i>N</i>-isopropylacrylamide) (PIPAAm)-grafted surface bound with heparin-binding epidermal growth factor-like growth factor (HB-EGF) was able to culture hepatocytes maintaining high albumin secretion and high expression of hepatocyte-specific genes. However, the activity of HB-EGF on the surface and its binding effects on hepatocytes remain unclear. In this study, we investigated the temperature-dependent interactions of HB-EGF and EGF receptor (EGFR) with heparin-modified PIPAAm to evaluate the activity of HB-EGF on the surface. Quartz crystal microbalance (QCM) measurements revealed that the amounts of adsorbed HB-EGF on either the heparin-modified PIPAAm-grafted surface (heparin-IC1) or PIPAAm-grafted surfaces were almost the same regardless of swelling/deswelli"],"journal":["RSC advances"],"pagination":["37225-37232"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9043771"],"repository":["biostudies-literature"],"pubmed_title":["Preservation of heparin-binding EGF-like growth factor activity on heparin-modified poly(<i>N</i>-isopropylacrylamide)-grafted surfaces."],"pmcid":["PMC9043771"],"pubmed_authors":["Yamato M","Yui N","Okano T","Kobayashi J","Arisaka Y"],"additional_accession":[]},"is_claimable":false,"name":"Preservation of heparin-binding EGF-like growth factor activity on heparin-modified poly(<i>N</i>-isopropylacrylamide)-grafted surfaces.","description":"A heparin-modified poly(<i>N</i>-isopropylacrylamide) (PIPAAm)-grafted surface bound with heparin-binding epidermal growth factor-like growth factor (HB-EGF) was able to culture hepatocytes maintaining high albumin secretion and high expression of hepatocyte-specific genes. However, the activity of HB-EGF on the surface and its binding effects on hepatocytes remain unclear. In this study, we investigated the temperature-dependent interactions of HB-EGF and EGF receptor (EGFR) with heparin-modified PIPAAm to evaluate the activity of HB-EGF on the surface. Quartz crystal microbalance (QCM) measurements revealed that the amounts of adsorbed HB-EGF on either the heparin-modified PIPAAm-grafted surface (heparin-IC1) or PIPAAm-grafted surfaces were almost the same regardless of swelling/deswelli","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-04-19T09:19:19.509Z","creation":"2025-04-19T09:19:19.509Z"},"accession":"S-EPMC9043771","cross_references":{"pubmed":["35496401"],"doi":["10.1039/d1ra07317f"]}}