biostudies-literatureUnknown11(59)Kobayashi JA heparin-modified poly(<i>N</i>-isopropylacrylamide) (PIPAAm)-grafted surface bound with heparin-binding epidermal growth factor-like growth factor (HB-EGF) was able to culture hepatocytes maintaining high albumin secretion and high expression of hepatocyte-specific genes. However, the activity of HB-EGF on the surface and its binding effects on hepatocytes remain unclear. In this study, we investigated the temperature-dependent interactions of HB-EGF and EGF receptor (EGFR) with heparin-modified PIPAAm to evaluate the activity of HB-EGF on the surface. Quartz crystal microbalance (QCM) measurements revealed that the amounts of adsorbed HB-EGF on either the heparin-modified PIPAAm-grafted surface (heparin-IC1) or PIPAAm-grafted surfaces were almost the same regardless of swelling/deswelliRSC advances37225-37232https://www.ebi.ac.uk/biostudies/studies/S-EPMC9043771biostudies-literaturePreservation of heparin-binding EGF-like growth factor activity on heparin-modified poly(<i>N</i>-isopropylacrylamide)-grafted surfaces.PMC9043771Yamato MYui NOkano TKobayashi JArisaka YfalsePreservation of heparin-binding EGF-like growth factor activity on heparin-modified poly(<i>N</i>-isopropylacrylamide)-grafted surfaces.A heparin-modified poly(<i>N</i>-isopropylacrylamide) (PIPAAm)-grafted surface bound with heparin-binding epidermal growth factor-like growth factor (HB-EGF) was able to culture hepatocytes maintaining high albumin secretion and high expression of hepatocyte-specific genes. However, the activity of HB-EGF on the surface and its binding effects on hepatocytes remain unclear. In this study, we investigated the temperature-dependent interactions of HB-EGF and EGF receptor (EGFR) with heparin-modified PIPAAm to evaluate the activity of HB-EGF on the surface. Quartz crystal microbalance (QCM) measurements revealed that the amounts of adsorbed HB-EGF on either the heparin-modified PIPAAm-grafted surface (heparin-IC1) or PIPAAm-grafted surfaces were almost the same regardless of swelling/deswelli2021-01-01T00:00:00Z2021 Nov2025-04-19T09:19:19.509Z2025-04-19T09:19:19.509ZS-EPMC90437713549640110.1039/d1ra07317f