biostudies-literatureDeng YGuangzhou Science and Technology Plan38219-38234https://www.ebi.ac.uk/biostudies/studies/S-EPMC9044015biostudies-literatureUnknown11(60)To increase the production of exopolysaccharides (EPS) and expand the application of <i>Schizophyllum commune</i> (<i>S. commune</i>) fermentation liquid, the traditional Chinese medicine Radix Puerariae (RP) with outstanding biological activity was selected as a culture additive to improve the EPS yield and enhance the antioxidant activity of fermented products from <i>S. commune</i>. The effects of three independent factors: A: initial pH (5.0-6.0), B: concentration of RP (10-14 g L^-1), and C: inoculum size (8-12%, v/v) on the EPS yield were evaluated. The results of response surface methodology (RSM) showed that the optimal fermentation conditions were: A: 5.40, B: 12.80 g L^-1, and C: 10.0%. The optimal yield of EPS was 8.41 ± 0.12 mg mL^-1, which showed RSC advancesEnhanced exopolysaccharide yield and antioxidant activities of <i>Schizophyllum commune</i> fermented products by the addition of Radix Puerariae.PMC9044015202103000055Zhou LZheng BLu DHu LGuo CHuang QLiu TDeng YfalseEnhanced exopolysaccharide yield and antioxidant activities of <i>Schizophyllum commune</i> fermented products by the addition of Radix Puerariae.To increase the production of exopolysaccharides (EPS) and expand the application of <i>Schizophyllum commune</i> (<i>S. commune</i>) fermentation liquid, the traditional Chinese medicine Radix Puerariae (RP) with outstanding biological activity was selected as a culture additive to improve the EPS yield and enhance the antioxidant activity of fermented products from <i>S. commune</i>. The effects of three independent factors: A: initial pH (5.0-6.0), B: concentration of RP (10-14 g L^-1), and C: inoculum size (8-12%, v/v) on the EPS yield were evaluated. The results of response surface methodology (RSM) showed that the optimal fermentation conditions were: A: 5.40, B: 12.80 g L^-1, and C: 10.0%. The optimal yield of EPS was 8.41 ± 0.12 mg mL^-1, which showed 2021-01-01T00:00:00Z2021 Nov2025-04-05T14:43:17.778Z2025-04-05T14:43:17.778ZS-EPMC90440153549808110.1039/d1ra06314f