biostudies-literatureChen HHong Kong General Research FundTong-ren-tang Pharmaceutical Inc. LTDShenzhen Basic Research Plan Project51https://www.ebi.ac.uk/biostudies/studies/S-EPMC9044615biostudies-literatureUnknown17(1)<h4>Background</h4>Hemorrhagic transformation (HT) is a common complication of delayed tissue plasminogen activator (t-PA) treatment for ischemic stroke. Peroxynitrite plays an important role in the breakdown of blood-brain barrier (BBB) and the development of HT. We tested the hypothesis that Angong Niuhuang Wan (AGNHW), a traditional Chinese medicinal formula, could be used in conjunction with t-PA to protect the BBB, minimize HT, and improve neurological function by suppressing peroxynitrite-mediated matrix metalloproteinase-9 (MMP-9) activation.<h4>Methods</h4>We first performed quality control study and chemical identification of AGNHW by using UPLC. In animal experiments, male Sprague-Dawley rats were subjected to 5 h of middle cerebral artery occlusion (MCAO) followed by 19 h of repChinese medicineAngong Niuhuang Wan reduces hemorrhagic transformation and mortality in ischemic stroke rats with delayed thrombolysis: involvement of peroxynitrite-mediated MMP-9 activation.PMC9044615SIRI/04/04/2015/0617118717AoE/P-705/16 Areas of Excellence Scheme17105220Gu BShen JTsoi BLuo YChen HQi SfalseAngong Niuhuang Wan reduces hemorrhagic transformation and mortality in ischemic stroke rats with delayed thrombolysis: involvement of peroxynitrite-mediated MMP-9 activation.<h4>Background</h4>Hemorrhagic transformation (HT) is a common complication of delayed tissue plasminogen activator (t-PA) treatment for ischemic stroke. Peroxynitrite plays an important role in the breakdown of blood-brain barrier (BBB) and the development of HT. We tested the hypothesis that Angong Niuhuang Wan (AGNHW), a traditional Chinese medicinal formula, could be used in conjunction with t-PA to protect the BBB, minimize HT, and improve neurological function by suppressing peroxynitrite-mediated matrix metalloproteinase-9 (MMP-9) activation.<h4>Methods</h4>We first performed quality control study and chemical identification of AGNHW by using UPLC. In animal experiments, male Sprague-Dawley rats were subjected to 5 h of middle cerebral artery occlusion (MCAO) followed by 19 h of rep2022-01-01T00:00:00Z2022 Apr2025-04-05T11:59:40.82Z2025-04-05T11:59:40.82ZS-EPMC90446153547757610.1186/s13020-022-00595-7