{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fang Q"],"funding":["National Natural Science Foundation of China"],"pagination":["e0008022"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9044933"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(2)"],"pubmed_abstract":["We isolated and characterized a novel phage from hospital sewage, P13, able to lyse ST11 K47 carbapenem-resistant Klebsiella pneumoniae (CRKP), a major CRKP lineage. P13 formed a large lytic plaque (3.0 to 6.0 mm in diameter) in double-layer LB agar after overnight coculture with its host bacterial strain. A translucent halo formed when the culture was prolonged to 48 h. P13 showed a narrow host range only lysing ST11 K47 CRKP with a burst size of around 167 PFU/cell and exhibited broad pH and thermal stability. Genome sequencing showed that P13 contains no virulence, lysogenic or antimicrobial resistance genes, making this lytic phage a potential agent for phage therapy. Transmission electron microscopy showed that P13 exhibited typical morphology of the family <i>Podoviridae</i> with an "],"journal":["mSphere"],"pubmed_title":["Lytic Phages against ST11 K47 Carbapenem-Resistant Klebsiella pneumoniae and the Corresponding Phage Resistance Mechanisms."],"pmcid":["PMC9044933"],"funding_grant_id":["81861138055"],"pubmed_authors":["Fang Q","Zong Z"],"additional_accession":[]},"is_claimable":false,"name":"Lytic Phages against ST11 K47 Carbapenem-Resistant Klebsiella pneumoniae and the Corresponding Phage Resistance Mechanisms.","description":"We isolated and characterized a novel phage from hospital sewage, P13, able to lyse ST11 K47 carbapenem-resistant Klebsiella pneumoniae (CRKP), a major CRKP lineage. P13 formed a large lytic plaque (3.0 to 6.0 mm in diameter) in double-layer LB agar after overnight coculture with its host bacterial strain. A translucent halo formed when the culture was prolonged to 48 h. P13 showed a narrow host range only lysing ST11 K47 CRKP with a burst size of around 167 PFU/cell and exhibited broad pH and thermal stability. Genome sequencing showed that P13 contains no virulence, lysogenic or antimicrobial resistance genes, making this lytic phage a potential agent for phage therapy. Transmission electron microscopy showed that P13 exhibited typical morphology of the family <i>Podoviridae</i> with an ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-06-07T07:32:22.025Z","creation":"2025-04-04T21:00:26.074Z"},"accession":"S-EPMC9044933","cross_references":{"pubmed":["35255715"],"doi":["10.1128/msphere.00080-22"]}}