biostudies-literatureZhang XEcological Adaptation and Molecular Basis of spreading epidemic of Major biological hazards in Foreste0258078https://www.ebi.ac.uk/biostudies/studies/S-EPMC9049501biostudies-literatureUnknown17(4)<h4>Objective</h4>A type of rodent called a zokor causes great harm to agriculture and forestry production due to its large and sophisticated diet. As this rodent lives subterrane for most of its life, researchers know little about its dietary habits. Further understanding of its diet is important for developing green and sustainable control strategies for the zokor.<h4>Methods</h4>Chloroplast trnL gene and internal transcription spacer 1 primers were selected for high-throughput sequencing of stomach contents of captured zokor by DNA metabarcoding.<h4>Results</h4>A total of 25 zokors were selected, the food list of 32 families, 80 genera, and 154 species was obtained. At the family level, it was found that zokors mainly fed on Asteraceae, Poaceae, Rosaceae, Pinaceae, Brassicaceae, and ApiPloS oneDNA metabarcoding uncovers the diet of subterranean rodents in China.PMC90495012017YFD0600103–4-3Zou XZou YZhang XNan XHan CXu ZfalseDNA metabarcoding uncovers the diet of subterranean rodents in China.<h4>Objective</h4>A type of rodent called a zokor causes great harm to agriculture and forestry production due to its large and sophisticated diet. As this rodent lives subterrane for most of its life, researchers know little about its dietary habits. Further understanding of its diet is important for developing green and sustainable control strategies for the zokor.<h4>Methods</h4>Chloroplast trnL gene and internal transcription spacer 1 primers were selected for high-throughput sequencing of stomach contents of captured zokor by DNA metabarcoding.<h4>Results</h4>A total of 25 zokors were selected, the food list of 32 families, 80 genera, and 154 species was obtained. At the family level, it was found that zokors mainly fed on Asteraceae, Poaceae, Rosaceae, Pinaceae, Brassicaceae, and Api2022-01-01T00:00:00Z20222026-05-31T06:06:23.057Z2025-02-19T01:55:27.154ZS-EPMC90495013548278110.1371/journal.pone.0258078