biostudies-literatureAriantari NPDeutscher Akademischer AustauschdienstDeutsche ForschungsgemeinschaftJürgen Manchot Stiftung7232-7240https://www.ebi.ac.uk/biostudies/studies/S-EPMC9049863biostudies-literatureUnknown10(12)Didymellanosine (1), the first analogue of the decahydrofluorene-class of natural products bearing a 13-membered macrocyclic alkaloid conjugated with adenosine, and a new benzolactone derivative, ascolactone C (4) along with eight known compounds (2, 3, 5-10), were isolated from a solid rice fermentation of the endophytic fungus <i>Didymella</i> sp. IEA-3B.1 derived from the host plant <i>Terminalia catappa</i>. In addition, ascochitamine (11) was obtained when (NH₄)₂SO₄ was added to rice medium and is reported here for the first time as a natural product. Didymellanosine (1) displayed strong activity against the murine lymphoma cell line L5178Y, Burkitt's lymphoma B cells (Ramos) and adult lymphoblastic leukemia T cells (Jurkat J16), with IC₅₀ vRSC advancesDidymellanosine, a new decahydrofluorene analogue, and ascolactone C from <i>Didymella</i> sp. IEA-3B.1, an endophyte of <i>Terminalia catappa</i>.PMC9049863270650915/GRK 2158Teusch NMuller WEGReimche IAriantari NPAncheeva EGroner YMeier DLiu ZWesselborg SKalscheuer RFrank MStuhldreier FProksch PfalseDidymellanosine, a new decahydrofluorene analogue, and ascolactone C from <i>Didymella</i> sp. IEA-3B.1, an endophyte of <i>Terminalia catappa</i>.Didymellanosine (1), the first analogue of the decahydrofluorene-class of natural products bearing a 13-membered macrocyclic alkaloid conjugated with adenosine, and a new benzolactone derivative, ascolactone C (4) along with eight known compounds (2, 3, 5-10), were isolated from a solid rice fermentation of the endophytic fungus <i>Didymella</i> sp. IEA-3B.1 derived from the host plant <i>Terminalia catappa</i>. In addition, ascochitamine (11) was obtained when (NH₄)₂SO₄ was added to rice medium and is reported here for the first time as a natural product. Didymellanosine (1) displayed strong activity against the murine lymphoma cell line L5178Y, Burkitt's lymphoma B cells (Ramos) and adult lymphoblastic leukemia T cells (Jurkat J16), with IC₅₀ v2020-01-01T00:00:00Z2020 Feb2026-05-31T06:06:18.985Z2025-02-19T01:55:30.119ZS-EPMC90498633549389410.1039/c9ra10685e