biostudies-literatureUnknown13Alves FHorizon 2020The recent increase in community-acquired <i>Clostridioides difficile</i> infections discloses the shift in this bacterium epidemiology. This study aimed at establishing a transmission network involving One Health components, as well as assessing the zoonotic potential and genomic features of dominant clones. Samples were collected from different compartments of animal, human and environmental origin, from an animal production unit. <i>C. difficile</i> isolates were characterized for toxigenic profile by multiplex-PCR, while genetic diversity was evaluated by PCR-ribotyping and whole genome-based analysis. The overall <i>C. difficile</i> prevalence was 37.2% (70/188), and included samples from environmental (58.3%, 35/60) and animal (31.5%, 35/111) compartments; human samples (<i>n</i> = 1Frontiers in microbiology858310https://www.ebi.ac.uk/biostudies/studies/S-EPMC9050547biostudies-literatureAssessment of the Transmission Dynamics of <i>Clostridioides difficile</i> in a Farm Environment Reveals the Presence of a New Toxigenic Strain Connected to Swine Production.PMC9050547Alves FMatias ROleastro MMoreira OGomes JPSequeira ASilveira LNunes ARodrigues JCCastro RfalseAssessment of the Transmission Dynamics of <i>Clostridioides difficile</i> in a Farm Environment Reveals the Presence of a New Toxigenic Strain Connected to Swine Production.The recent increase in community-acquired <i>Clostridioides difficile</i> infections discloses the shift in this bacterium epidemiology. This study aimed at establishing a transmission network involving One Health components, as well as assessing the zoonotic potential and genomic features of dominant clones. Samples were collected from different compartments of animal, human and environmental origin, from an animal production unit. <i>C. difficile</i> isolates were characterized for toxigenic profile by multiplex-PCR, while genetic diversity was evaluated by PCR-ribotyping and whole genome-based analysis. The overall <i>C. difficile</i> prevalence was 37.2% (70/188), and included samples from environmental (58.3%, 35/60) and animal (31.5%, 35/111) compartments; human samples (<i>n</i> = 12022-01-01T00:00:00Z20222026-04-08T17:39:32.426Z2022-07-12T10:41:23.631ZS-EPMC90505473549567910.3389/fmicb.2022.858310