{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Munden A"],"funding":["National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["6903"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9050644"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Replication of the eukaryotic genome requires the formation of thousands of replication forks that must work in concert to accurately replicate the genetic and epigenetic information. Defining replication fork-associated proteins is a key step in understanding how genomes are replicated and repaired in the context of chromatin to maintain genome stability. To identify replication fork-associated proteins, we performed iPOND (Isolation of Proteins on Nascent DNA) coupled to quantitative mass spectrometry in Drosophila embryos and cultured cells. We identified 76 and 278 fork-associated proteins in post-MZT embryos and Drosophila cultured S2 cells, respectively. By performing a targeted screen of a subset of these proteins, we demonstrate that BRWD3, a targeting specificity factor for the DD"],"journal":["Scientific reports"],"pubmed_title":["Identification of replication fork-associated proteins in Drosophila embryos and cultured cells using iPOND coupled to quantitative mass spectrometry."],"pmcid":["PMC9050644"],"funding_grant_id":["R35 GM133552","R35 GM128650","R35GM128650","R35GM133552","T32 GM065086"],"pubmed_authors":["Munden A","Wright MT","Nordman JT","Plate L","Han D","Tirgar R"],"additional_accession":[]},"is_claimable":false,"name":"Identification of replication fork-associated proteins in Drosophila embryos and cultured cells using iPOND coupled to quantitative mass spectrometry.","description":"Replication of the eukaryotic genome requires the formation of thousands of replication forks that must work in concert to accurately replicate the genetic and epigenetic information. Defining replication fork-associated proteins is a key step in understanding how genomes are replicated and repaired in the context of chromatin to maintain genome stability. To identify replication fork-associated proteins, we performed iPOND (Isolation of Proteins on Nascent DNA) coupled to quantitative mass spectrometry in Drosophila embryos and cultured cells. We identified 76 and 278 fork-associated proteins in post-MZT embryos and Drosophila cultured S2 cells, respectively. By performing a targeted screen of a subset of these proteins, we demonstrate that BRWD3, a targeting specificity factor for the DD","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-05-18T12:06:19.141Z","creation":"2025-04-06T19:14:53.686Z"},"accession":"S-EPMC9050644","cross_references":{"pubmed":["35484306"],"doi":["10.1038/s41598-022-10821-9"]}}