biostudies-literatureWang SNational Institute of General Medical Sciences20073-20082https://www.ebi.ac.uk/biostudies/studies/S-EPMC9054207biostudies-literatureUnknown10(34)The Src homology 2 domain containing protein tyrosine phosphatase-2 (SHP2) is a key enzyme in pathways regulating tumor growth signaling, and recently gained interest as a promising anticancer drug target. Many SHP2 inhibitors are currently under development, including SHP099, which has shown potent anticancer activity at low concentrations <i>in vivo</i>. In this work, we developed multilayer coatings for localized delivery of SHP099 to improve upon current cancer therapies. Layer-by-layer self-assembly was used to develop films composed of chitosan and poly-carboxymethyl-β-cyclodextrin (PβCD) for the delivery of SHP099. SHP099 was successfully loaded into multilayer films <i>via</i> host-guest interactions with PβCD. Nuclear magnetic resonance spectroscopy confirmed the occurrence of thiRSC advancesControlled delivery of a protein tyrosine phosphatase inhibitor, SHP099, using cyclodextrin-mediated host-guest interactions in polyelectrolyte multilayer films for cancer therapy.PMC9054207U54GM115677Yang WAntoci VWang SAlkekhia DShukla ABattigelli AFairman AMoore DfalseControlled delivery of a protein tyrosine phosphatase inhibitor, SHP099, using cyclodextrin-mediated host-guest interactions in polyelectrolyte multilayer films for cancer therapy.The Src homology 2 domain containing protein tyrosine phosphatase-2 (SHP2) is a key enzyme in pathways regulating tumor growth signaling, and recently gained interest as a promising anticancer drug target. Many SHP2 inhibitors are currently under development, including SHP099, which has shown potent anticancer activity at low concentrations <i>in vivo</i>. In this work, we developed multilayer coatings for localized delivery of SHP099 to improve upon current cancer therapies. Layer-by-layer self-assembly was used to develop films composed of chitosan and poly-carboxymethyl-β-cyclodextrin (PβCD) for the delivery of SHP099. SHP099 was successfully loaded into multilayer films <i>via</i> host-guest interactions with PβCD. Nuclear magnetic resonance spectroscopy confirmed the occurrence of thi2020-01-01T00:00:00Z2020 May2025-04-18T14:13:42.261Z2025-04-04T20:21:56.906ZS-EPMC90542073552044110.1039/d0ra03864d