biostudies-literatureXing YYNational Natural Science Foundation of China26151-26164https://www.ebi.ac.uk/biostudies/studies/S-EPMC9055353biostudies-literatureUnknown10(44)In this study, a novel polysaccharide was isolated from <i>Artemisia ordosica</i> by water-extraction-ethanol-precipitation method. The optimal extraction conditions of <i>Artemisia ordosica</i> polysaccharide (AOP) were determined by single factor investigation and response surface methodology optimization, and were shown as follows: a liquid-solid ratio of 15.4 : 1 mL g^-1, extraction time of 4.3 h, extraction temperature of 60 °C. Under the optimal conditions, the extraction yield and the sugar content of the AOP were 5.56% and 52.65%. Gel permeation chromatography coupled to multi-angle laser light scattering, a refractive index detection system and ion-exchange chromatography were used to determine the characterization of AOP. These results indicated that AOP, with a moleculRSC advancesOptimization extraction and characterization of <i>Artemisia ordosica</i> polysaccharide and its beneficial effects on antioxidant function and gut microbiota in rats.PMC905535331960667Shi LLJin XXu YQXing YYGuo SWShi BLYan SMfalseOptimization extraction and characterization of <i>Artemisia ordosica</i> polysaccharide and its beneficial effects on antioxidant function and gut microbiota in rats.In this study, a novel polysaccharide was isolated from <i>Artemisia ordosica</i> by water-extraction-ethanol-precipitation method. The optimal extraction conditions of <i>Artemisia ordosica</i> polysaccharide (AOP) were determined by single factor investigation and response surface methodology optimization, and were shown as follows: a liquid-solid ratio of 15.4 : 1 mL g^-1, extraction time of 4.3 h, extraction temperature of 60 °C. Under the optimal conditions, the extraction yield and the sugar content of the AOP were 5.56% and 52.65%. Gel permeation chromatography coupled to multi-angle laser light scattering, a refractive index detection system and ion-exchange chromatography were used to determine the characterization of AOP. These results indicated that AOP, with a molecul2020-01-01T00:00:00Z2020 Jul2025-04-22T05:51:14.684Z2025-04-05T21:28:02.274ZS-EPMC90553533551975110.1039/d0ra05063f