{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kohler T"],"funding":["Technische Universität Dresden","Bundesministerium für Bildung und Forschung","Deutsche Forschungsgemeinschaft","European Research Council"],"pagination":["40588-40596"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9057574"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(66)"],"pubmed_abstract":["In the present work, microgels were utilized as a cell-free reaction environment to produce a functional malonyl-CoA synthetase (deGFP-MatB) under geometry-controlled transcription and translation. Our approach combines the straight-forward optimization of overall protein yield of an <i>E. coli</i>-based cell-free protein synthesis (CFPS) system based on concentration screening of magnesium and potassium glutamate, DNA as well as polyethylene glycol (PEG), and its innovative usage in microgel-based production of a key enzyme of the polyketide synthesis pathway. After partial modification of the carboxyl groups of hyaluronic acid (HA) with 5'-methylfuran groups <i>via</i> 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM)-activation, these were further functionalize"],"journal":["RSC advances"],"pubmed_title":["Cell-free protein synthesis and <i>in situ</i> immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion."],"pmcid":["PMC9057574"],"funding_grant_id":["GRK 1865","031A360C","852065","TH 2037/1-1"],"pubmed_authors":["Weigel N","Thiele J","Hoefgen S","Kohler T","Heida T","Valiante V"],"additional_accession":[]},"is_claimable":false,"name":"Cell-free protein synthesis and <i>in situ</i> immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion.","description":"In the present work, microgels were utilized as a cell-free reaction environment to produce a functional malonyl-CoA synthetase (deGFP-MatB) under geometry-controlled transcription and translation. Our approach combines the straight-forward optimization of overall protein yield of an <i>E. coli</i>-based cell-free protein synthesis (CFPS) system based on concentration screening of magnesium and potassium glutamate, DNA as well as polyethylene glycol (PEG), and its innovative usage in microgel-based production of a key enzyme of the polyketide synthesis pathway. After partial modification of the carboxyl groups of hyaluronic acid (HA) with 5'-methylfuran groups <i>via</i> 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM)-activation, these were further functionalize","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Nov","modification":"2025-04-19T09:20:15.651Z","creation":"2025-04-19T09:20:15.651Z"},"accession":"S-EPMC9057574","cross_references":{"pubmed":["35520868"],"doi":["10.1039/d0ra06702d"]}}