biostudies-literatureYe YPearl River S and T Nova Program of GuangzhouNatural Science Foundation of Guangdong Province44-51https://www.ebi.ac.uk/biostudies/studies/S-EPMC9059480biostudies-literatureUnknown9(1)The purpose of the present study is to evaluate the preparation and the structure of fraction OP-Ia and its protective effect against UV-induced photoaging through the MAPKs signaling pathway. Fractions OP-Ia and OP-Ib were prepared by enzymatic hydrolysis and purified by ultrafiltration (5 kDa) and gel chromatography (Sephadex G-25). The reducing power and superoxide radical scavenging ability were evaluated, which showed that OP-Ia had stronger antioxidant activity than OP-Ib. Next, ten peptides were identified in OP-Ia by UPLC-MS/MS. The mechanism of the anti-photoaging activity for fraction OP-Ia was investigated through the MAPKs pathway based on the HaCaT cell line. Fraction OP-Ia could inhibit the generation of ROS and the decline of cell viability induced by UV radiation, meanwhileRSC advancesPreparation, structure identification and the anti-photoaging activity of peptide fraction OP-Ia from <i>Ostrea rivularis</i>.PMC90594802016100100962016A030312001You LLi XZhao MDeng QYe YfalsePreparation, structure identification and the anti-photoaging activity of peptide fraction OP-Ia from <i>Ostrea rivularis</i>.The purpose of the present study is to evaluate the preparation and the structure of fraction OP-Ia and its protective effect against UV-induced photoaging through the MAPKs signaling pathway. Fractions OP-Ia and OP-Ib were prepared by enzymatic hydrolysis and purified by ultrafiltration (5 kDa) and gel chromatography (Sephadex G-25). The reducing power and superoxide radical scavenging ability were evaluated, which showed that OP-Ia had stronger antioxidant activity than OP-Ib. Next, ten peptides were identified in OP-Ia by UPLC-MS/MS. The mechanism of the anti-photoaging activity for fraction OP-Ia was investigated through the MAPKs pathway based on the HaCaT cell line. Fraction OP-Ia could inhibit the generation of ROS and the decline of cell viability induced by UV radiation, meanwhile2018-01-01T00:00:00Z2018 Dec2025-04-19T09:54:17.256Z2025-04-19T09:54:17.256ZS-EPMC90594803552159510.1039/c8ra08137a