{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ramirez RN"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["eabj9836"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9059705"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(67)"],"pubmed_abstract":["Gene expression programs are specified by higher-order chromatin structure and enhancer-promoter loops (EPLs). T regulatory cell (T<sub>reg</sub>) identity is dominantly specified by the transcription factor (TF) FoxP3, whose mechanism of action is unclear. We applied chromatin conformation capture with immunoprecipitation (HiChIP) in T<sub>reg</sub> and closely related conventional CD4<sup>+</sup> T cells (T<sub>conv</sub>). EPLs identified by H3K27Ac HiChIP showed a range of connection intensity, with some superconnected genes. TF-specific HiChIP showed that FoxP3 interacts with EPLs at a large number of genes, including some not differentially expressed in T<sub>reg</sub> versus T<sub>conv</sub>, but enriched at the core T<sub>reg</sub> signature loci that it up-regulates. FoxP3 associa"],"journal":["Science immunology"],"pubmed_title":["FoxP3 associates with enhancer-promoter loops to regulate T<sub>reg</sub>-specific gene expression."],"pmcid":["PMC9059705"],"funding_grant_id":["T32 GM007753","R01 AI116834","R01 AI150686"],"pubmed_authors":["Leon J","Ramirez RN","Mathis D","Benoist C","Chowdhary K"],"additional_accession":[]},"is_claimable":false,"name":"FoxP3 associates with enhancer-promoter loops to regulate T<sub>reg</sub>-specific gene expression.","description":"Gene expression programs are specified by higher-order chromatin structure and enhancer-promoter loops (EPLs). T regulatory cell (T<sub>reg</sub>) identity is dominantly specified by the transcription factor (TF) FoxP3, whose mechanism of action is unclear. We applied chromatin conformation capture with immunoprecipitation (HiChIP) in T<sub>reg</sub> and closely related conventional CD4<sup>+</sup> T cells (T<sub>conv</sub>). EPLs identified by H3K27Ac HiChIP showed a range of connection intensity, with some superconnected genes. TF-specific HiChIP showed that FoxP3 interacts with EPLs at a large number of genes, including some not differentially expressed in T<sub>reg</sub> versus T<sub>conv</sub>, but enriched at the core T<sub>reg</sub> signature loci that it up-regulates. FoxP3 associa","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-04T20:35:32.413Z","creation":"2025-04-04T20:35:32.413Z"},"accession":"S-EPMC9059705","cross_references":{"pubmed":["35030035"],"doi":["10.1126/sciimmunol.abj9836"]}}