biostudies-literatureChen YShanghai Municipal Education CommissionShanghai Institute of Technology2325-2331https://www.ebi.ac.uk/biostudies/studies/S-EPMC9059822biostudies-literatureUnknown9(4)The production of (<i>S</i>)-<i>N</i>-boc-3-hydroxy piperidine (NBHP) <i>via</i> asymmetric bioreduction of 1-boc-3-piperidinone with reductase is impeded by the need for expensive coenzymes NAD(P)H. In order to regenerate the coenzyme <i>in situ</i>, the gene of alcohol dehydrogenase from <i>Thermoanaerobacter brockii</i> and glucose dehydrogenase from <i>Bacillus subtilis</i> were ligated into the multiple cloning sites of pRSFDuet-1 plasmid to construct the recombinant <i>Escherichia</i> BL21 (DE3) that co-expressing alcohol dehydrogenase and glucose dehydrogenase. Different culture conditions including the medium composition, inducer and pH <i>etc</i> were systematically investigated to improve the enzyme production. The enzyme activity was increased more than 11-fold under optimal culRSC advancesEfficient synthesis of Ibrutinib chiral intermediate in high space-time yield by recombinant <i>E. coli</i> co-expressing alcohol dehydrogenase and glucose dehydrogenase.PMC9059822No. ZZyyx15011No. YJ2015-36Ma BCao SChen YWu XXu YfalseEfficient synthesis of Ibrutinib chiral intermediate in high space-time yield by recombinant <i>E. coli</i> co-expressing alcohol dehydrogenase and glucose dehydrogenase.The production of (<i>S</i>)-<i>N</i>-boc-3-hydroxy piperidine (NBHP) <i>via</i> asymmetric bioreduction of 1-boc-3-piperidinone with reductase is impeded by the need for expensive coenzymes NAD(P)H. In order to regenerate the coenzyme <i>in situ</i>, the gene of alcohol dehydrogenase from <i>Thermoanaerobacter brockii</i> and glucose dehydrogenase from <i>Bacillus subtilis</i> were ligated into the multiple cloning sites of pRSFDuet-1 plasmid to construct the recombinant <i>Escherichia</i> BL21 (DE3) that co-expressing alcohol dehydrogenase and glucose dehydrogenase. Different culture conditions including the medium composition, inducer and pH <i>etc</i> were systematically investigated to improve the enzyme production. The enzyme activity was increased more than 11-fold under optimal cul2019-01-01T00:00:00Z2019 Jan2025-05-18T12:06:57.788Z2025-05-18T12:06:57.788ZS-EPMC90598223551611410.1039/c8ra08100j