{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Brusatori M"],"funding":["Community Foundation for Southeastern Michigan","NCRR NIH HHS","NIH, National Center for Research Resources","Paul Strauss Endowed Chair and Wayne State University SSIM Program","National Institutes of Health","Wayne State University"],"pagination":["271-281"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9060146"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(4)"],"pubmed_abstract":["<h4>Background</h4>This study profiles ceramides extracted from visceral and subcutaneous adipose tissue of human subjects by liquid chromatography-mass spectrometry to determine a correlation with status of diabetes and gender.<h4>Methods</h4>Samples of visceral and abdominal wall subcutaneous adipose tissue (n = 36 and n = 31, respectively) were taken during laparoscopic surgery from 36 patients (14 nondiabetic, 22 diabetic and prediabetic) undergoing bariatric surgery with a body mass index (BMI) >35 kg/m<sup>2</sup> with ≥1 existing comorbidity or BMI ≥40 kg/m<sup>2</sup> . Sphingolipids were extracted and analyzed using liquid chromatography-mass spectrometry.<h4>Results</h4>After logarithm 2 conversion, paired analysis of visceral to subcutaneous tissue showed differential accumulation of Cer(d18:1/16:0), Cer(d18:1/18:0), and Cer(d18:1/24:1) in visceral tissue of prediabetic/diabetic female subjects, but not in males. Within-tissue analysis showed higher mean levels of ceramide species linked to insulin resistance, such as Cer(d18:1/18:0) and Cer(d18:1/16:0), in visceral tissue of prediabetic/diabetic patients compared with nondiabetic subjects and higher content of Cer(d18:1/14:0) in subcutaneous tissue of insulin-resistant female patients compared with prediabetic/diabetic males. Statistically significant differences in mean levels of ceramide species between insulin-resistant African American and insulin-resistant Caucasian patients were not evident in visceral or subcutaneous tissue.<h4>Conclusions</h4>Analysis of ceramides is important for developing a better understanding of biological processes underlying type 2 diabetes, metabolic syndrome, and obesity. Knowledge of the accumulated ceramides/dihydroceramides may reflect on the prelipolytic state that leads the lipotoxic phase of insulin resistance and may shed light on the predisposition to insulin resistance by gender."],"journal":["Journal of diabetes"],"pubmed_title":["Ceramide changes in abdominal subcutaneous and visceral adipose tissue among diabetic and nondiabetic patients."],"pmcid":["PMC9060146"],"funding_grant_id":["S10RR027926","S10 RR027926"],"pubmed_authors":["Seyoum B","Auner GW","Brusatori M","Tucker SC","Wood MH","Maddipati KR","Koya SK","Honn KV"],"additional_accession":[]},"is_claimable":false,"name":"Ceramide changes in abdominal subcutaneous and visceral adipose tissue among diabetic and nondiabetic patients.","description":"<h4>Background</h4>This study profiles ceramides extracted from visceral and subcutaneous adipose tissue of human subjects by liquid chromatography-mass spectrometry to determine a correlation with status of diabetes and gender.<h4>Methods</h4>Samples of visceral and abdominal wall subcutaneous adipose tissue (n = 36 and n = 31, respectively) were taken during laparoscopic surgery from 36 patients (14 nondiabetic, 22 diabetic and prediabetic) undergoing bariatric surgery with a body mass index (BMI) >35 kg/m<sup>2</sup> with ≥1 existing comorbidity or BMI ≥40 kg/m<sup>2</sup> . Sphingolipids were extracted and analyzed using liquid chromatography-mass spectrometry.<h4>Results</h4>After logarithm 2 conversion, paired analysis of visceral to subcutaneous tissue showed differential accumulation of Cer(d18:1/16:0), Cer(d18:1/18:0), and Cer(d18:1/24:1) in visceral tissue of prediabetic/diabetic female subjects, but not in males. Within-tissue analysis showed higher mean levels of ceramide species linked to insulin resistance, such as Cer(d18:1/18:0) and Cer(d18:1/16:0), in visceral tissue of prediabetic/diabetic patients compared with nondiabetic subjects and higher content of Cer(d18:1/14:0) in subcutaneous tissue of insulin-resistant female patients compared with prediabetic/diabetic males. Statistically significant differences in mean levels of ceramide species between insulin-resistant African American and insulin-resistant Caucasian patients were not evident in visceral or subcutaneous tissue.<h4>Conclusions</h4>Analysis of ceramides is important for developing a better understanding of biological processes underlying type 2 diabetes, metabolic syndrome, and obesity. Knowledge of the accumulated ceramides/dihydroceramides may reflect on the prelipolytic state that leads the lipotoxic phase of insulin resistance and may shed light on the predisposition to insulin resistance by gender.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-04T19:42:19.476Z","creation":"2025-04-04T19:42:19.476Z"},"accession":"S-EPMC9060146","cross_references":{"pubmed":["35470585"],"doi":["10.1111/1753-0407.13262"]}}