{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Peng L"],"funding":["National Natural Science Foundation of China"],"pagination":["5295-5301"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9060652"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(10)"],"pubmed_abstract":["In the present study, an extraction method, combining extraction by ethyl acetate + ethanol and purification by HPD400 resin, was established to obtain huangjiu polyphenol extract (HPE). After extraction and purification, the polyphenol yield was 22.57%, and 90.57% protein and 97.99% sugar were removed. HPLC analysis indicated that (+)-catechin (91.33 μg mL<sup>-1</sup>) was the predominant phenolic compound among the 11 detected polyphenols. In LPS-stimulated RAW264.7 cells, HPE exhibits anti-inflammatory effects by inhibiting the production of NO and pro-inflammatory cytokines (TNF-α, interleukin IL-6 and IL-1β). The anti-inflammatory effect of HPE is associated with the inhibition of iNOS expression, the suppression of NF-κB translocation to the nucleus, and the inhibition of the phosphorylation of IκB and the MAPK family proteins, <i>e.g.</i> p-38, Erk 1/2, and JNK. Moreover, the activation of Nrf2 and HO-1 is also related to the anti-inflammatory effect of HPE."],"journal":["RSC advances"],"pubmed_title":["Polyphenols extracted from huangjiu have anti-inflammatory activity in lipopolysaccharide stimulated RAW264.7 cells."],"pmcid":["PMC9060652"],"funding_grant_id":["31571823","31701593","31771968"],"pubmed_authors":["Ai-Lati A","Ji Z","Peng L","Chen S","Mao J"],"additional_accession":[]},"is_claimable":false,"name":"Polyphenols extracted from huangjiu have anti-inflammatory activity in lipopolysaccharide stimulated RAW264.7 cells.","description":"In the present study, an extraction method, combining extraction by ethyl acetate + ethanol and purification by HPD400 resin, was established to obtain huangjiu polyphenol extract (HPE). After extraction and purification, the polyphenol yield was 22.57%, and 90.57% protein and 97.99% sugar were removed. HPLC analysis indicated that (+)-catechin (91.33 μg mL<sup>-1</sup>) was the predominant phenolic compound among the 11 detected polyphenols. In LPS-stimulated RAW264.7 cells, HPE exhibits anti-inflammatory effects by inhibiting the production of NO and pro-inflammatory cytokines (TNF-α, interleukin IL-6 and IL-1β). The anti-inflammatory effect of HPE is associated with the inhibition of iNOS expression, the suppression of NF-κB translocation to the nucleus, and the inhibition of the phosphorylation of IκB and the MAPK family proteins, <i>e.g.</i> p-38, Erk 1/2, and JNK. Moreover, the activation of Nrf2 and HO-1 is also related to the anti-inflammatory effect of HPE.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Feb","modification":"2025-04-04T21:31:35.244Z","creation":"2025-04-04T21:31:35.244Z"},"accession":"S-EPMC9060652","cross_references":{"pubmed":["35515913"],"doi":["10.1039/c8ra09671f"]}}