biostudies-literatureUnknown8(1)Rick TIn bacteria, the monopolar localization of enzymes and protein complexes can result in a bimodal distribution of enzyme activity between the dividing cells and heterogeneity of cellular behaviors. In Shewanella putrefaciens, the multidomain hybrid diguanylate cyclase/phosphodiesterase PdeB, which degrades the secondary messenger c-di-GMP, is located at the flagellated cell pole. Here, we show that direct interaction between the inactive diguanylate cyclase (GGDEF) domain of PdeB and the FimV domain of the polar landmark protein HubP is crucial for full function of PdeB as a phosphodiesterase. Thus, the GGDEF domain serves as a spatially controlled on-switch that effectively restricts PdeBs activity to the flagellated cell pole. PdeB regulates abundance and activity of at least two crucial NPJ biofilms and microbiomes35https://www.ebi.ac.uk/biostudies/studies/S-EPMC9061725biostudies-literatureGGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP.PMC9061725Steinchen WKnauer SKSeifert RHochberg GFiedler SGlatter TBange GGraumann PLRick TBahre HThormann KMHoing AKreiling VfalseGGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP.In bacteria, the monopolar localization of enzymes and protein complexes can result in a bimodal distribution of enzyme activity between the dividing cells and heterogeneity of cellular behaviors. In Shewanella putrefaciens, the multidomain hybrid diguanylate cyclase/phosphodiesterase PdeB, which degrades the secondary messenger c-di-GMP, is located at the flagellated cell pole. Here, we show that direct interaction between the inactive diguanylate cyclase (GGDEF) domain of PdeB and the FimV domain of the polar landmark protein HubP is crucial for full function of PdeB as a phosphodiesterase. Thus, the GGDEF domain serves as a spatially controlled on-switch that effectively restricts PdeBs activity to the flagellated cell pole. PdeB regulates abundance and activity of at least two crucial 2022-01-01T00:00:00Z2022 May2025-04-25T18:06:44.378Z2025-04-25T18:06:44.378ZS-EPMC90617253550142410.1038/s41522-022-00297-w