biostudies-literatureUnknown61Li BYNational Key Research and Development Program of China Stem Cell and Translational ResearchNational Natural Science Foundation of China<h4>Objective</h4>Due to the increasing prevalence of obesity and insulin resistance, there is an urgent need for better treatment of obesity and its related metabolic disorders. This study aimed to elucidate the role of SERPINA3C, an adipocyte secreted protein, in obesity and related metabolic disorders.<h4>Methods</h4>Male wild type (WT) and knockout (KO) mice were fed with high-fat diet (HFD) for 16 weeks, adiposity, insulin resistance, and inflammation were assessed. AAV-mediated overexpression of SERPINA3C was injected locally in inguinal white adipose tissue (iWAT) to examine the effect of SERPINA3C. In vitro analyses were conducted in 3T3-L1 adipocytes to explore the molecular pathways underlying the function of SERPINA3C.<h4>Results</h4>Functional exploration of the SERPINA3C knockMolecular metabolism101500https://www.ebi.ac.uk/biostudies/studies/S-EPMC9062745biostudies-literatureSERPINA3C ameliorates adipose tissue inflammation through the Cathepsin G/Integrin/AKT pathway.PMC9062745Guo LGuo YYTang QQLi BYXiao GfalseSERPINA3C ameliorates adipose tissue inflammation through the Cathepsin G/Integrin/AKT pathway.<h4>Objective</h4>Due to the increasing prevalence of obesity and insulin resistance, there is an urgent need for better treatment of obesity and its related metabolic disorders. This study aimed to elucidate the role of SERPINA3C, an adipocyte secreted protein, in obesity and related metabolic disorders.<h4>Methods</h4>Male wild type (WT) and knockout (KO) mice were fed with high-fat diet (HFD) for 16 weeks, adiposity, insulin resistance, and inflammation were assessed. AAV-mediated overexpression of SERPINA3C was injected locally in inguinal white adipose tissue (iWAT) to examine the effect of SERPINA3C. In vitro analyses were conducted in 3T3-L1 adipocytes to explore the molecular pathways underlying the function of SERPINA3C.<h4>Results</h4>Functional exploration of the SERPINA3C knock2022-01-01T00:00:00Z2022 Jul2025-04-25T18:02:58.819Z2025-04-25T18:02:58.819ZS-EPMC90627453543658710.1016/j.molmet.2022.101500