biostudies-literatureMuhammad ISpecial Fund for Agro-scientific Research in the Public InterestEducation Department of Jilin ProvinceNational Natural Science Foundation of China14758-14765https://www.ebi.ac.uk/biostudies/studies/S-EPMC9064161biostudies-literatureUnknown9(26)Outbreaks of cobweb disease are becoming increasingly prevalent globally, severely affecting the quality and yield of <i>Agaricus bisporus</i>. However, cobweb disease-resistant strains are rare, and little is known regarding the biocontrol management of the disease. Here, we isolated a pathogen from a severe outbreak of cobweb disease on <i>A. bisporus</i> in China and identified it as <i>Cladobotryum mycophilum</i> based on morphological characteristics, rDNA sequences, and pathogenicity tests. We then tested 30 <i>A. bisporus</i> strains for cobweb disease resistance by inoculating with <i>C. mycophilum</i> and evaluated the activity of different botanicals. We found that two wild strains of <i>A. bisporus</i> originating from the Tibetan Plateau in China were resistant to cobweb diseasRSC advancesIdentification of resistance to cobweb disease caused by <i>Cladobotryum mycophilum</i> in wild and cultivated strains of <i>Agaricus bisporus</i> and screening for bioactive botanicals.PMC906416120150313731701977Sossah FLLi YYang YLi SLi DFu YMuhammad IfalseIdentification of resistance to cobweb disease caused by <i>Cladobotryum mycophilum</i> in wild and cultivated strains of <i>Agaricus bisporus</i> and screening for bioactive botanicals.Outbreaks of cobweb disease are becoming increasingly prevalent globally, severely affecting the quality and yield of <i>Agaricus bisporus</i>. However, cobweb disease-resistant strains are rare, and little is known regarding the biocontrol management of the disease. Here, we isolated a pathogen from a severe outbreak of cobweb disease on <i>A. bisporus</i> in China and identified it as <i>Cladobotryum mycophilum</i> based on morphological characteristics, rDNA sequences, and pathogenicity tests. We then tested 30 <i>A. bisporus</i> strains for cobweb disease resistance by inoculating with <i>C. mycophilum</i> and evaluated the activity of different botanicals. We found that two wild strains of <i>A. bisporus</i> originating from the Tibetan Plateau in China were resistant to cobweb diseas2019-01-01T00:00:00Z2019 May2026-05-30T14:50:10.824Z2022-07-11T20:05:26.062ZS-EPMC90641613551634310.1039/c9ra00632j