biostudies-literatureUnknown9(3)Xuan L<h4>Objective</h4>As a common complication of coronary microembolization (CME), myocardial injury (MI) implies high mortality. Long non-coding RNAs (lncRNAs) are rarely studied in CME-induced MI. Herein, this study intended to evaluate the role of lncRNA Sox2 overlapping transcript (Sox2OT) in CME-induced MI.<h4>Methods</h4>The CME rat models were successfully established by injection of microemboli. Rat cardiac functions and MI were observed by ultrasonic electrocardiogram, HE staining, and HBFP staining. Functional assays were utilized to test the inflammatory responses, oxidative stress, and pyroptosis using reverse transcription quantitative polymerase chain reaction, Western blotting, immunohistochemistry, immunofluorescence, and ELISA. Dual-luciferase reporter gene assay and RNA immuESC heart failure1689-1702https://www.ebi.ac.uk/biostudies/studies/S-EPMC9065873biostudies-literatureLong non-coding RNA Sox2OT promotes coronary microembolization-induced myocardial injury by mediating pyroptosis.PMC9065873Bai DFu DYu LZhen DXuan LGong GfalseLong non-coding RNA Sox2OT promotes coronary microembolization-induced myocardial injury by mediating pyroptosis.<h4>Objective</h4>As a common complication of coronary microembolization (CME), myocardial injury (MI) implies high mortality. Long non-coding RNAs (lncRNAs) are rarely studied in CME-induced MI. Herein, this study intended to evaluate the role of lncRNA Sox2 overlapping transcript (Sox2OT) in CME-induced MI.<h4>Methods</h4>The CME rat models were successfully established by injection of microemboli. Rat cardiac functions and MI were observed by ultrasonic electrocardiogram, HE staining, and HBFP staining. Functional assays were utilized to test the inflammatory responses, oxidative stress, and pyroptosis using reverse transcription quantitative polymerase chain reaction, Western blotting, immunohistochemistry, immunofluorescence, and ELISA. Dual-luciferase reporter gene assay and RNA immu2022-01-01T00:00:00Z2022 Jun2025-04-22T07:51:59.231Z2025-04-05T22:18:08.324ZS-EPMC90658733530483410.1002/ehf2.13814