{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao Z"],"funding":["National Natural Science Foundation of China","Science and Technology development Planning Project of Jilin Province"],"pagination":["794-807"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9067532"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["The immunogenicity of Aha1, an OMP of <i>Aeromonas hydrophila</i> mediating the adhesion of bacteria onto the mucosal surface of hosts has been established. In this study, recombinant vectors, pPG1 and pPG2, carrying a 1366 bp DNA fragment that was responsible for encoding the 49 kDa Aha1 from <i>A. hydrophila</i> were constructed, respectively, then electroporated into a probiotic strain <i>Lactobacillus casei</i> CC16 separately to generate two types of recombinants, <i>L. casei-</i>pPG1-Aha1 (Lc-pPG1-Aha1) and <i>L. casei</i>-pPG2-Aha1 (Lc-pPG2-Aha1). Subsequently, these were orally administered into common carps to examine their immunogenicity. The expression and localization of the expressed Aha1 protein relative to the carrier <i>L. casei</i> was validated via Western blotting, flow "],"journal":["Virulence"],"pubmed_title":["Oral vaccination with recombinant <i>Lactobacillus casei</i> expressing <i>Aeromonas hydrophila</i> Aha1 against <i>A. hydrophila</i> infections in common carps."],"pmcid":["PMC9067532"],"funding_grant_id":["20210202049NC","32102681"],"pubmed_authors":["Guan Y","Zhang D","Cong B","Siddiqui SA","Feng-Shan X","Wang H","Zhao Z"],"additional_accession":[]},"is_claimable":false,"name":"Oral vaccination with recombinant <i>Lactobacillus casei</i> expressing <i>Aeromonas hydrophila</i> Aha1 against <i>A. hydrophila</i> infections in common carps.","description":"The immunogenicity of Aha1, an OMP of <i>Aeromonas hydrophila</i> mediating the adhesion of bacteria onto the mucosal surface of hosts has been established. In this study, recombinant vectors, pPG1 and pPG2, carrying a 1366 bp DNA fragment that was responsible for encoding the 49 kDa Aha1 from <i>A. hydrophila</i> were constructed, respectively, then electroporated into a probiotic strain <i>Lactobacillus casei</i> CC16 separately to generate two types of recombinants, <i>L. casei-</i>pPG1-Aha1 (Lc-pPG1-Aha1) and <i>L. casei</i>-pPG2-Aha1 (Lc-pPG2-Aha1). Subsequently, these were orally administered into common carps to examine their immunogenicity. The expression and localization of the expressed Aha1 protein relative to the carrier <i>L. casei</i> was validated via Western blotting, flow ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-18T12:13:08.94Z","creation":"2025-04-06T21:51:03.067Z"},"accession":"S-EPMC9067532","cross_references":{"pubmed":["35499101"],"doi":["10.1080/21505594.2022.2063484"]}}