biostudies-literatureZhao ZNational Natural Science Foundation of ChinaScience and Technology development Planning Project of Jilin Province794-807https://www.ebi.ac.uk/biostudies/studies/S-EPMC9067532biostudies-literatureUnknown13(1)The immunogenicity of Aha1, an OMP of <i>Aeromonas hydrophila</i> mediating the adhesion of bacteria onto the mucosal surface of hosts has been established. In this study, recombinant vectors, pPG1 and pPG2, carrying a 1366 bp DNA fragment that was responsible for encoding the 49 kDa Aha1 from <i>A. hydrophila</i> were constructed, respectively, then electroporated into a probiotic strain <i>Lactobacillus casei</i> CC16 separately to generate two types of recombinants, <i>L. casei-</i>pPG1-Aha1 (Lc-pPG1-Aha1) and <i>L. casei</i>-pPG2-Aha1 (Lc-pPG2-Aha1). Subsequently, these were orally administered into common carps to examine their immunogenicity. The expression and localization of the expressed Aha1 protein relative to the carrier <i>L. casei</i> was validated via Western blotting, flow VirulenceOral vaccination with recombinant <i>Lactobacillus casei</i> expressing <i>Aeromonas hydrophila</i> Aha1 against <i>A. hydrophila</i> infections in common carps.PMC906753220210202049NC32102681Guan YZhang DCong BSiddiqui SAFeng-Shan XWang HZhao ZfalseOral vaccination with recombinant <i>Lactobacillus casei</i> expressing <i>Aeromonas hydrophila</i> Aha1 against <i>A. hydrophila</i> infections in common carps.The immunogenicity of Aha1, an OMP of <i>Aeromonas hydrophila</i> mediating the adhesion of bacteria onto the mucosal surface of hosts has been established. In this study, recombinant vectors, pPG1 and pPG2, carrying a 1366 bp DNA fragment that was responsible for encoding the 49 kDa Aha1 from <i>A. hydrophila</i> were constructed, respectively, then electroporated into a probiotic strain <i>Lactobacillus casei</i> CC16 separately to generate two types of recombinants, <i>L. casei-</i>pPG1-Aha1 (Lc-pPG1-Aha1) and <i>L. casei</i>-pPG2-Aha1 (Lc-pPG2-Aha1). Subsequently, these were orally administered into common carps to examine their immunogenicity. The expression and localization of the expressed Aha1 protein relative to the carrier <i>L. casei</i> was validated via Western blotting, flow 2022-01-01T00:00:00Z2022 Dec2025-04-18T12:13:08.94Z2025-04-06T21:51:03.067ZS-EPMC90675323549910110.1080/21505594.2022.2063484