{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kho S"],"funding":["Bill & Melinda Gates Foundation"],"pagination":["7286"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9068623"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Commercial point-of-care tests remain insufficient for accurately detecting and differentiating low-level malaria infections in regions co-endemic with multiple non-falciparum species, including zoonotic Plasmodium knowlesi (Pk). A 5-plex chemiluminescent assay simultaneously measures pan-Plasmodium lactate dehydrogenase (pLDH), P. falciparum (Pf)-LDH, P. vivax (Pv)-LDH, Pf-histidine-rich protein-2 (HRP2), and C-reactive protein. We assessed its diagnostic performance on whole blood (WB) samples from 102 healthy controls and 306 PCR-confirmed clinical cases of Pf, Pv, Pk, P. malariae (Pm) and P. ovale (Po) mono-infections from Southeast-Asia. We confirm its excellent HRP2-based detection of Pf. Cross-reactivity of Pf-LDH with all non-falciparum species tested was observed (specificity 57.3"],"journal":["Scientific reports"],"pubmed_title":["Diagnostic performance of a 5-plex malaria immunoassay in regions co-endemic for Plasmodium falciparum, P. vivax, P. knowlesi, P. malariae and P. ovale."],"pmcid":["PMC9068623"],"funding_grant_id":["INV1135840"],"pubmed_authors":["Jang IK","Kenangalem E","Kho S","Domingo GJ","Piera K","McCarthy JS","William T","Anstey NM","Britton S","Barber BE","Grigg MJ"],"additional_accession":[]},"is_claimable":false,"name":"Diagnostic performance of a 5-plex malaria immunoassay in regions co-endemic for Plasmodium falciparum, P. vivax, P. knowlesi, P. malariae and P. ovale.","description":"Commercial point-of-care tests remain insufficient for accurately detecting and differentiating low-level malaria infections in regions co-endemic with multiple non-falciparum species, including zoonotic Plasmodium knowlesi (Pk). A 5-plex chemiluminescent assay simultaneously measures pan-Plasmodium lactate dehydrogenase (pLDH), P. falciparum (Pf)-LDH, P. vivax (Pv)-LDH, Pf-histidine-rich protein-2 (HRP2), and C-reactive protein. We assessed its diagnostic performance on whole blood (WB) samples from 102 healthy controls and 306 PCR-confirmed clinical cases of Pf, Pv, Pk, P. malariae (Pm) and P. ovale (Po) mono-infections from Southeast-Asia. We confirm its excellent HRP2-based detection of Pf. Cross-reactivity of Pf-LDH with all non-falciparum species tested was observed (specificity 57.3","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-18T15:43:54.787Z","creation":"2025-02-19T04:21:47.253Z"},"accession":"S-EPMC9068623","cross_references":{"pubmed":["35508558"],"doi":["10.1038/s41598-022-11042-w"]}}