biostudies-literatureYao DTexas AgriLife ResearchNIGMS NIH HHSNational Science Foundation2088-2103https://www.ebi.ac.uk/biostudies/studies/S-EPMC9070964biostudies-literatureUnknown14(12)Poly(ADP-ribosyl)ation (PARylation) is a posttranslational modification reversibly catalyzed by poly(ADP-ribose) polymerases (PARPs) and poly(ADP-ribose) glycohydrolases (PARGs) and plays a key role in multiple cellular processes. The molecular mechanisms by which PARylation regulates innate immunity remain largely unknown in eukaryotes. Here we show that Arabidopsis UBC13A and UBC13B, the major drivers of lysine 63 (K63)-linked polyubiquitination, directly interact with PARPs/PARGs. Activation of pathogen-associated molecular pattern (PAMP)-triggered immunity promotes these interactions and enhances PARylation of UBC13. Both parp1 parp2 and ubc13a ubc13b mutants are compromised in immune responses with increased accumulation of total pathogenesis-related (PR) proteins but decreased accumuMolecular plantCoordinated regulation of plant immunity by poly(ADP-ribosyl)ation and K63-linked ubiquitination.PMC9070964R01 GM092893IOS-1951094Bent AFArguez MAYao DSong JHe PfalseCoordinated regulation of plant immunity by poly(ADP-ribosyl)ation and K63-linked ubiquitination.Poly(ADP-ribosyl)ation (PARylation) is a posttranslational modification reversibly catalyzed by poly(ADP-ribose) polymerases (PARPs) and poly(ADP-ribose) glycohydrolases (PARGs) and plays a key role in multiple cellular processes. The molecular mechanisms by which PARylation regulates innate immunity remain largely unknown in eukaryotes. Here we show that Arabidopsis UBC13A and UBC13B, the major drivers of lysine 63 (K63)-linked polyubiquitination, directly interact with PARPs/PARGs. Activation of pathogen-associated molecular pattern (PAMP)-triggered immunity promotes these interactions and enhances PARylation of UBC13. Both parp1 parp2 and ubc13a ubc13b mutants are compromised in immune responses with increased accumulation of total pathogenesis-related (PR) proteins but decreased accumu2021-01-01T00:00:00Z2021 Dec2025-04-05T15:58:37.717Z2025-04-05T15:58:37.717ZS-EPMC90709643441855110.1016/j.molp.2021.08.013