{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Soudet J"],"funding":["Swiss National Science Foundation"],"pagination":["4515-4528"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9071385"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(8)"],"pubmed_abstract":["Eukaryotic genomes are pervasively transcribed by RNA polymerase II (RNAPII), and transcription of long non-coding RNAs often overlaps with coding gene promoters. This might lead to coding gene repression in a process named Transcription Interference (TI). In Saccharomyces cerevisiae, TI is mainly driven by antisense non-coding transcription and occurs through re-shaping of promoter Nucleosome-Depleted Regions (NDRs). In this study, we developed a genetic screen to identify new players involved in Antisense-Mediated Transcription Interference (AMTI). Among the candidates, we found the HIR histone chaperone complex known to be involved in de novo histone deposition. Using genome-wide approaches, we reveal that HIR-dependent histone deposition represses the promoters of SAGA-dependent genes "],"journal":["Nucleic acids research"],"pubmed_title":["Antisense-mediated repression of SAGA-dependent genes involves the HIR histone chaperone."],"pmcid":["PMC9071385"],"funding_grant_id":["31003A_182344","153331","182344","31003A_153331"],"pubmed_authors":["Pastucha AM","Maffioletti A","Soudet J","Bakir Z","Beyrouthy N","Menendez D","Stutz F"],"additional_accession":[]},"is_claimable":false,"name":"Antisense-mediated repression of SAGA-dependent genes involves the HIR histone chaperone.","description":"Eukaryotic genomes are pervasively transcribed by RNA polymerase II (RNAPII), and transcription of long non-coding RNAs often overlaps with coding gene promoters. This might lead to coding gene repression in a process named Transcription Interference (TI). In Saccharomyces cerevisiae, TI is mainly driven by antisense non-coding transcription and occurs through re-shaping of promoter Nucleosome-Depleted Regions (NDRs). In this study, we developed a genetic screen to identify new players involved in Antisense-Mediated Transcription Interference (AMTI). Among the candidates, we found the HIR histone chaperone complex known to be involved in de novo histone deposition. Using genome-wide approaches, we reveal that HIR-dependent histone deposition represses the promoters of SAGA-dependent genes ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2026-05-19T03:12:47.261Z","creation":"2025-04-04T07:56:20.982Z"},"accession":"S-EPMC9071385","cross_references":{"pubmed":["35474134"],"doi":["10.1093/nar/gkac264"]}}