biostudies-literatureSoudet JSwiss National Science Foundation4515-4528https://www.ebi.ac.uk/biostudies/studies/S-EPMC9071385biostudies-literatureUnknown50(8)Eukaryotic genomes are pervasively transcribed by RNA polymerase II (RNAPII), and transcription of long non-coding RNAs often overlaps with coding gene promoters. This might lead to coding gene repression in a process named Transcription Interference (TI). In Saccharomyces cerevisiae, TI is mainly driven by antisense non-coding transcription and occurs through re-shaping of promoter Nucleosome-Depleted Regions (NDRs). In this study, we developed a genetic screen to identify new players involved in Antisense-Mediated Transcription Interference (AMTI). Among the candidates, we found the HIR histone chaperone complex known to be involved in de novo histone deposition. Using genome-wide approaches, we reveal that HIR-dependent histone deposition represses the promoters of SAGA-dependent genes Nucleic acids researchAntisense-mediated repression of SAGA-dependent genes involves the HIR histone chaperone.PMC907138531003A_18234415333118234431003A_153331Pastucha AMMaffioletti ASoudet JBakir ZBeyrouthy NMenendez DStutz FfalseAntisense-mediated repression of SAGA-dependent genes involves the HIR histone chaperone.Eukaryotic genomes are pervasively transcribed by RNA polymerase II (RNAPII), and transcription of long non-coding RNAs often overlaps with coding gene promoters. This might lead to coding gene repression in a process named Transcription Interference (TI). In Saccharomyces cerevisiae, TI is mainly driven by antisense non-coding transcription and occurs through re-shaping of promoter Nucleosome-Depleted Regions (NDRs). In this study, we developed a genetic screen to identify new players involved in Antisense-Mediated Transcription Interference (AMTI). Among the candidates, we found the HIR histone chaperone complex known to be involved in de novo histone deposition. Using genome-wide approaches, we reveal that HIR-dependent histone deposition represses the promoters of SAGA-dependent genes 2022-01-01T00:00:00Z2022 May2026-05-19T03:12:47.261Z2025-04-04T07:56:20.982ZS-EPMC90713853547413410.1093/nar/gkac264