biostudies-literatureYe ZHNational Natural Science Foundation of China8513747https://www.ebi.ac.uk/biostudies/studies/S-EPMC9071859biostudies-literatureUnknown2022<h4>Background</h4>Neoadjuvant immunotherapy is promising for locally advanced non-small-cell lung cancer (NSCLC). The <i>in situ</i> immune patterns, as a predictor of PD-1/PD-L1 blockade outcomes, of the primary tumor (PT) and metastatic lymph nodes (mLNs) are unknown.<h4>Methods</h4>Multiplex immunofluorescence staining and multispectral imaging were used to evaluate the <i>in situ</i> immune patterns of T cells (CD3+) and cytotoxic T cells (CD8+) in terms of density, location (center of tumor (CT) and invasive margin (IM)), and the PD-L1 expression status of tumor cells and stromal T cells of paired PTs and mLNs in 38 stage III NSCLCs.<h4>Results</h4>The densities of T cells and cytotoxic T cells were correlated between PTs and mLNs at both CT and IM. Higher densities of stromal T cellJournal of immunology researchDifferent <i>In Situ</i> Immune Patterns between Primary Tumor and Lymph Node in Non-Small-Cell Lung Cancer: Potential Impact on Neoadjuvant Immunotherapy.PMC907185982002407Long HYe ZHZhao ZRfalseDifferent <i>In Situ</i> Immune Patterns between Primary Tumor and Lymph Node in Non-Small-Cell Lung Cancer: Potential Impact on Neoadjuvant Immunotherapy.<h4>Background</h4>Neoadjuvant immunotherapy is promising for locally advanced non-small-cell lung cancer (NSCLC). The <i>in situ</i> immune patterns, as a predictor of PD-1/PD-L1 blockade outcomes, of the primary tumor (PT) and metastatic lymph nodes (mLNs) are unknown.<h4>Methods</h4>Multiplex immunofluorescence staining and multispectral imaging were used to evaluate the <i>in situ</i> immune patterns of T cells (CD3+) and cytotoxic T cells (CD8+) in terms of density, location (center of tumor (CT) and invasive margin (IM)), and the PD-L1 expression status of tumor cells and stromal T cells of paired PTs and mLNs in 38 stage III NSCLCs.<h4>Results</h4>The densities of T cells and cytotoxic T cells were correlated between PTs and mLNs at both CT and IM. Higher densities of stromal T cell2022-01-01T00:00:00Z20222025-05-31T23:12:08.456Z2024-11-12T02:28:07.812ZS-EPMC90718593552861510.1155/2022/8513747