biostudies-literatureUnknown2022Nagarajan KAntibiotic resistance represents one of the biggest challenges, and there is an urgent need for plant-based antimicrobial agents that enable managing this crisis effectively. In this work, we aimed to investigate the antibacterial activity of <i>Astragalus candolleanus</i> (<i>A. candolleanus</i>) hydromethanolic root extract against Gram-positive (<i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>) and Gram-negative (<i>Escherichia coli</i>, <i>Salmonella typhimurium</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, and <i>Kocuria rhizophila</i>) strains by the cup-plate method. The root was powdered and extracted with 70% methanol by cold maceration for 5 days. Preliminary phytochemical screening was performed with different solvents in the order of increasing polaInternational journal of microbiology4584799https://www.ebi.ac.uk/biostudies/studies/S-EPMC9072053biostudies-literatureIdentification of Potent Bioassay Guided Terpenoid and Glycoside Root Fractions of <i>Astragalus candolleanus</i> against Clinically Significant Bacterial Strains.PMC9072053Goel RGhai RGenovese CPrasad TKalaivani MTeotia AKNagarajan KD'Angeli FGrover PVarshney GfalseIdentification of Potent Bioassay Guided Terpenoid and Glycoside Root Fractions of <i>Astragalus candolleanus</i> against Clinically Significant Bacterial Strains.Antibiotic resistance represents one of the biggest challenges, and there is an urgent need for plant-based antimicrobial agents that enable managing this crisis effectively. In this work, we aimed to investigate the antibacterial activity of <i>Astragalus candolleanus</i> (<i>A. candolleanus</i>) hydromethanolic root extract against Gram-positive (<i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>) and Gram-negative (<i>Escherichia coli</i>, <i>Salmonella typhimurium</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, and <i>Kocuria rhizophila</i>) strains by the cup-plate method. The root was powdered and extracted with 70% methanol by cold maceration for 5 days. Preliminary phytochemical screening was performed with different solvents in the order of increasing pola2022-01-01T00:00:00Z20222025-04-04T07:57:04.542Z2024-11-09T03:42:01.709ZS-EPMC90720533552831310.1155/2022/4584799