{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Joseph CR"],"funding":["Associazione Italiana per la Ricerca sul Cancro","European Research Council","EC | Horizon 2020 Framework Programme"],"pagination":["2480"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9072374"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["DNA damage tolerance (DDT), activated by replication stress during genome replication, is mediated by translesion synthesis and homologous recombination (HR). Here we uncover that DDK kinase, essential for replication initiation, is critical for replication-associated recombination-mediated DDT. DDK relies on its multi-monoSUMOylation to facilitate HR-mediated DDT and optimal retention of Rad51 recombinase at replication damage sites. Impairment of DDK kinase activity, reduced monoSUMOylation and mutations in the putative SUMO Interacting Motifs (SIMs) of Rad51 impair replication-associated recombination and cause fork uncoupling with accumulation of large single-stranded DNA regions at fork branching points. Notably, genetic activation of salvage recombination rescues the uncoupled fork p"],"journal":["Nature communications"],"pubmed_title":["Rad51-mediated replication of damaged templates relies on monoSUMOylated DDK kinase."],"pmcid":["PMC9072374"],"funding_grant_id":["682190","23710","18976"],"pubmed_authors":["Branzei D","Joseph CR","Dusi S","Giannattasio M"],"additional_accession":[]},"is_claimable":false,"name":"Rad51-mediated replication of damaged templates relies on monoSUMOylated DDK kinase.","description":"DNA damage tolerance (DDT), activated by replication stress during genome replication, is mediated by translesion synthesis and homologous recombination (HR). Here we uncover that DDK kinase, essential for replication initiation, is critical for replication-associated recombination-mediated DDT. DDK relies on its multi-monoSUMOylation to facilitate HR-mediated DDT and optimal retention of Rad51 recombinase at replication damage sites. Impairment of DDK kinase activity, reduced monoSUMOylation and mutations in the putative SUMO Interacting Motifs (SIMs) of Rad51 impair replication-associated recombination and cause fork uncoupling with accumulation of large single-stranded DNA regions at fork branching points. Notably, genetic activation of salvage recombination rescues the uncoupled fork p","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-19T20:10:17.766Z","creation":"2025-04-19T20:10:17.766Z"},"accession":"S-EPMC9072374","cross_references":{"pubmed":["35513396"],"doi":["10.1038/s41467-022-30215-9"]}}