biostudies-literatureUnknown12Yan J<h4>Aim</h4>To investigate the treating effect of Yiqi-Bushen-Tiaozhi (YBT) recipe on nonalcoholic steatohepatitis (NASH) mice, determine whether the outcome was associated with gut microbiota, and clarify the regulating mechanism.<h4>Methods</h4>NASH mice were induced by high-fat and high-fructose diets (HFFD). In the fifth week, mice in the YBT group were orally administrated YBT (22.12g·kg^-1·d^-1) daily for 12 weeks. Fresh stool of mice was collected at the 16^th week for fecal 16S rDNA analysis. Hepatic pathology and biochemical indicators were used to reflect the improvement of YBT on hepatic inflammation and lipid metabolism in NASH mice. Quantitative real-time PCR (qRT-PCR) was used to verify the results of PICRUSt analysis.<h4>Results</h4>Results of tFrontiers in cellular and infection microbiology824597https://www.ebi.ac.uk/biostudies/studies/S-EPMC9072834biostudies-literatureYiqi-Bushen-Tiaozhi Recipe Attenuated High-Fat and High-Fructose Diet Induced Nonalcoholic Steatohepatitis in Mice <i>via</i> Gut Microbiota.PMC9072834Liu YYan JLi JWu LChen ZHe BNie YfalseYiqi-Bushen-Tiaozhi Recipe Attenuated High-Fat and High-Fructose Diet Induced Nonalcoholic Steatohepatitis in Mice <i>via</i> Gut Microbiota.<h4>Aim</h4>To investigate the treating effect of Yiqi-Bushen-Tiaozhi (YBT) recipe on nonalcoholic steatohepatitis (NASH) mice, determine whether the outcome was associated with gut microbiota, and clarify the regulating mechanism.<h4>Methods</h4>NASH mice were induced by high-fat and high-fructose diets (HFFD). In the fifth week, mice in the YBT group were orally administrated YBT (22.12g·kg^-1·d^-1) daily for 12 weeks. Fresh stool of mice was collected at the 16^th week for fecal 16S rDNA analysis. Hepatic pathology and biochemical indicators were used to reflect the improvement of YBT on hepatic inflammation and lipid metabolism in NASH mice. Quantitative real-time PCR (qRT-PCR) was used to verify the results of PICRUSt analysis.<h4>Results</h4>Results of t2022-01-01T00:00:00Z20222026-03-15T19:25:09.14Z2025-04-06T19:46:22.308ZS-EPMC90728343553133410.3389/fcimb.2022.824597