biostudies-literatureUnknown12(5)Ryu TOkinawa Institute of Science and Technology Graduate UniversityThe false clownfish Amphiprion ocellaris is a popular fish species and an emerging model organism for studying the ecology, evolution, adaptation, and developmental biology of reef fishes. Despite this, high-quality genomic resources for this species are scarce, hindering advanced genomic analyses. Leveraging the power of PacBio long-read sequencing and Hi-C chromosome conformation capture techniques, we constructed a high-quality chromosome-scale genome assembly for the clownfish A. ocellaris. The initial genome assembly comprised of 1,551 contigs of 861.42 Mb, with an N50 of 863.85 kb. Hi-C scaffolding of the genome resulted in 24 chromosomes containing 856.61 Mb. The genome was annotated with 26,797 protein-coding genes and had 96.62% completeness of conserved actinopterygian genes, makG3 (Bethesda, Md.)jkac074https://www.ebi.ac.uk/biostudies/studies/S-EPMC9073690biostudies-literatureA chromosome-scale genome assembly of the false clownfish, Amphiprion ocellaris.PMC9073690Ravasi TLaudet VMoore BIzumiyama MKawai EHerrera MRyu TfalseA chromosome-scale genome assembly of the false clownfish, Amphiprion ocellaris.The false clownfish Amphiprion ocellaris is a popular fish species and an emerging model organism for studying the ecology, evolution, adaptation, and developmental biology of reef fishes. Despite this, high-quality genomic resources for this species are scarce, hindering advanced genomic analyses. Leveraging the power of PacBio long-read sequencing and Hi-C chromosome conformation capture techniques, we constructed a high-quality chromosome-scale genome assembly for the clownfish A. ocellaris. The initial genome assembly comprised of 1,551 contigs of 861.42 Mb, with an N50 of 863.85 kb. Hi-C scaffolding of the genome resulted in 24 chromosomes containing 856.61 Mb. The genome was annotated with 26,797 protein-coding genes and had 96.62% completeness of conserved actinopterygian genes, mak2022-01-01T00:00:00Z2022 May2026-05-31T05:05:46.27Z2025-02-19T01:54:48.663ZS-EPMC90736903535319210.1093/g3journal/jkac074