{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pellegrinelli RP"],"funding":["Swiss National Science Foundation","?cole Polytechnique F?d?rale de Lausanne","European Research Council"],"pagination":["859-864"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9074103"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(5)"],"pubmed_abstract":["Determining the primary structure of glycans remains challenging due to their isomeric complexity. While high-resolution ion mobility spectrometry (IMS) has recently allowed distinguishing between many glycan isomers, the arrival-time distributions (ATDs) frequently exhibit multiple peaks, which can arise from positional isomers, reducing-end anomers, or different conformations. Here, we present the combination of ultrahigh-resolution ion mobility, collision-induced dissociation (CID), and cryogenic infrared (IR) spectroscopy as a systematic method to identify reducing-end anomers of glycans. Previous studies have suggested that high-resolution ion mobility of sodiated glycans is able to separate the two reducing-end anomers. In this case, Y-fragments generated from mobility-separated precursor species should also contain a single anomer at their reducing end. We confirm that this is the case by comparing the IR spectra of selected Y-fragments to those of anomerically pure mono- and disaccharides, allowing the assignment of the mobility-separated precursor and its IR spectrum to a single reducing-end anomer. The anomerically pure precursor glycans can henceforth be rapidly identified on the basis of their IR spectrum alone, allowing them to be distinguished from other isomeric forms."],"journal":["Journal of the American Society for Mass Spectrometry"],"pubmed_title":["A New Strategy Coupling Ion-Mobility-Selective CID and Cryogenic IR Spectroscopy to Identify Glycan Anomers."],"pmcid":["PMC9074103"],"funding_grant_id":["788697","206021_177004","200020_184838"],"pubmed_authors":["Pellegrinelli RP","Ben Faleh A","Warnke S","Yue L","Carrascosa E","Bansal P","Rizzo TR"],"additional_accession":[]},"is_claimable":false,"name":"A New Strategy Coupling Ion-Mobility-Selective CID and Cryogenic IR Spectroscopy to Identify Glycan Anomers.","description":"Determining the primary structure of glycans remains challenging due to their isomeric complexity. While high-resolution ion mobility spectrometry (IMS) has recently allowed distinguishing between many glycan isomers, the arrival-time distributions (ATDs) frequently exhibit multiple peaks, which can arise from positional isomers, reducing-end anomers, or different conformations. Here, we present the combination of ultrahigh-resolution ion mobility, collision-induced dissociation (CID), and cryogenic infrared (IR) spectroscopy as a systematic method to identify reducing-end anomers of glycans. Previous studies have suggested that high-resolution ion mobility of sodiated glycans is able to separate the two reducing-end anomers. In this case, Y-fragments generated from mobility-separated precursor species should also contain a single anomer at their reducing end. We confirm that this is the case by comparing the IR spectra of selected Y-fragments to those of anomerically pure mono- and disaccharides, allowing the assignment of the mobility-separated precursor and its IR spectrum to a single reducing-end anomer. The anomerically pure precursor glycans can henceforth be rapidly identified on the basis of their IR spectrum alone, allowing them to be distinguished from other isomeric forms.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-18T14:14:12.418Z","creation":"2025-04-04T20:22:05.384Z"},"accession":"S-EPMC9074103","cross_references":{"pubmed":["35437995"],"doi":["10.1021/jasms.2c00043"]}}