{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jones K"],"funding":["National Institute of Arthritis and Musculoskeletal and Skin Diseases","NIAMS NIH HHS"],"pagination":["789349"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9074688"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["SOX4 belongs to the group C of the SOX transcription factor family. It is a critical mediator of tumor necrosis factor alpha (TNF)-induced transformation of fibroblast-like s-ynoviocytes (FLS) in arthritis. In this study we investigated the genome wide association between the DNA binding and transcriptional activities of SOX4 and the NF-kappaB signaling transcription factor RELA/p65 downstream of TNF signaling. We used ChIP-seq assays in mouse FLS to compare the global DNA binding profiles of SOX4 and RELA. RNA-seq of TNF-induced wildtype and <i>SoxC</i>-knockout FLS was used to identify the SOX4-dependent and independent aspects of the TNF-regulated transcriptome. We found that SOX4 and RELA physically interact with each other on the chromatin. Interestingly, ChIP-seq assays revealed that"],"journal":["Frontiers in immunology"],"pubmed_title":["SOX4 and RELA Function as Transcriptional Partners to Regulate the Expression of TNF- Responsive Genes in Fibroblast-Like Synoviocytes."],"pmcid":["PMC9074688"],"funding_grant_id":["R01 AR070736"],"pubmed_authors":["Niu S","Jones K","Bhattaram P","Ramirez-Perez S","Drissi H","Gangishetti U"],"additional_accession":[]},"is_claimable":false,"name":"SOX4 and RELA Function as Transcriptional Partners to Regulate the Expression of TNF- Responsive Genes in Fibroblast-Like Synoviocytes.","description":"SOX4 belongs to the group C of the SOX transcription factor family. It is a critical mediator of tumor necrosis factor alpha (TNF)-induced transformation of fibroblast-like s-ynoviocytes (FLS) in arthritis. In this study we investigated the genome wide association between the DNA binding and transcriptional activities of SOX4 and the NF-kappaB signaling transcription factor RELA/p65 downstream of TNF signaling. We used ChIP-seq assays in mouse FLS to compare the global DNA binding profiles of SOX4 and RELA. RNA-seq of TNF-induced wildtype and <i>SoxC</i>-knockout FLS was used to identify the SOX4-dependent and independent aspects of the TNF-regulated transcriptome. We found that SOX4 and RELA physically interact with each other on the chromatin. Interestingly, ChIP-seq assays revealed that","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-07-14T16:44:55.02Z","creation":"2025-02-19T03:27:37.723Z"},"accession":"S-EPMC9074688","cross_references":{"pubmed":["35529852"],"doi":["10.3389/fimmu.2022.789349"]}}