biostudies-literatureUnknown9(71)Li JNatural Sciences and Engineering Research Council of CanadaNational Research Council CanadaBis-(3'-5')-cyclic dimeric 2'-deoxy-2'-fluoroguanosine monophosphate (2'-F-c-di-GMP) was synthesized through the modified H-phosphonate chemistry. Oral immunization of C57BL/6 mice with Helicobacter pylori cell-free sonicate extract adjuvanted with 2'-F-c-di-GMP led to the production of antigen-specific antibodies in feces and sera, and lowered bacterial counts in the stomach upon post-vaccination infections in immunized mice. Similarly, oral vaccination of BALB/c mice with flagillin proteins from Clostridium difficile and Listeria monocytogenes adjuvanted with 2'-F-c-di-GMP led to production of antigen-specific antibodies both systemically and mucosally. The adjuvanticity of 2'-F-c-di-GMP is associated with the enhanced induction of interferon γ. These results demonstRSC advances41481-41489https://www.ebi.ac.uk/biostudies/studies/S-EPMC9076492biostudies-literature2'-Fluoro-c-di-GMP as an oral vaccine adjuvant.PMC9076492Li JLee RKChen WYan Hfalse2'-Fluoro-c-di-GMP as an oral vaccine adjuvant.Bis-(3'-5')-cyclic dimeric 2'-deoxy-2'-fluoroguanosine monophosphate (2'-F-c-di-GMP) was synthesized through the modified H-phosphonate chemistry. Oral immunization of C57BL/6 mice with Helicobacter pylori cell-free sonicate extract adjuvanted with 2'-F-c-di-GMP led to the production of antigen-specific antibodies in feces and sera, and lowered bacterial counts in the stomach upon post-vaccination infections in immunized mice. Similarly, oral vaccination of BALB/c mice with flagillin proteins from Clostridium difficile and Listeria monocytogenes adjuvanted with 2'-F-c-di-GMP led to production of antigen-specific antibodies both systemically and mucosally. The adjuvanticity of 2'-F-c-di-GMP is associated with the enhanced induction of interferon γ. These results demonst2019-01-01T00:00:00Z2019 Dec2025-04-04T23:17:16.51Z2024-10-19T04:03:42.781ZS-EPMC90764923554160510.1039/c9ra08310c