{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yu J"],"funding":["Natural Science Foundation of Zhejiang Province","National Natural Science Foundation of China"],"pagination":["2357-2364"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9077389"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(5)"],"pubmed_abstract":["To develop an efficient and green immobilized biocatalyst for promiscuous catalysis which has a broad scope of applications, hybrid nanoflower (hNF) confined papain as a biocatalyst has been proposed and characterized in this study. hNFs were firstly prepared through mixing CuSO<sub>4</sub> aqueous solution with papain in phosphate saline (PBS) at room temperature. The resulting hNFs were characterized by SEM and verified through a hydrolysis reaction with <i>N</i>-benzoyl-dl-arginine amide as substrate. Under optimal conditions, this nano-biocatalyst demonstrated a 15-fold hydrolytic activity compared with papain of free form, along with better thermal stability. A series of reaction factors (reaction temperature, time, and solvent) have been investigated for Knoevenagel condensation reac"],"journal":["RSC advances"],"pubmed_title":["Efficient promiscuous Knoevenagel condensation catalyzed by papain confined in Cu<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> nanoflowers."],"pmcid":["PMC9077389"],"funding_grant_id":["LY18B060009","21576062","LY15B060011"],"pubmed_authors":["Wang A","Yang L","Wu SG","Pei X","Chen X","Yu J","Jiang M","Zhang P"],"additional_accession":[]},"is_claimable":false,"name":"Efficient promiscuous Knoevenagel condensation catalyzed by papain confined in Cu<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> nanoflowers.","description":"To develop an efficient and green immobilized biocatalyst for promiscuous catalysis which has a broad scope of applications, hybrid nanoflower (hNF) confined papain as a biocatalyst has been proposed and characterized in this study. hNFs were firstly prepared through mixing CuSO<sub>4</sub> aqueous solution with papain in phosphate saline (PBS) at room temperature. The resulting hNFs were characterized by SEM and verified through a hydrolysis reaction with <i>N</i>-benzoyl-dl-arginine amide as substrate. Under optimal conditions, this nano-biocatalyst demonstrated a 15-fold hydrolytic activity compared with papain of free form, along with better thermal stability. A series of reaction factors (reaction temperature, time, and solvent) have been investigated for Knoevenagel condensation reac","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Jan","modification":"2025-04-18T20:24:11.279Z","creation":"2025-04-07T08:22:22.026Z"},"accession":"S-EPMC9077389","cross_references":{"pubmed":["35541490"],"doi":["10.1039/c7ra12940h"]}}