biostudies-literatureUnknown2022Wang HEmerging studies have proved that tRNA-derived fragments (tRFs) play vital roles in tumor metastasis; however, the function of tRFs in gastric cancer (GC) remains largely unclear. We investigated the role of tRF-24-V29K9UV3IU in growth and metastasis of GC using a xenograft mouse model. Differential gene expression downstream of tRF-24-V29K9UV3IU was identified by transcriptome sequencing, and interaction was then verified by a dual luciferase reporter and RNA immunoprecipitation. MKN-45 cells were also used to explore the biological functions of tRF-24-V29K9UV3IU <i>in vitro</i>. Here, knockdown of tRF-24-V29K9UV3IU promoted tumor growth and metastasis of GC <i>in vivo</i>. The expression of tRF-24-V29K9UV3IU and E-cadherin (epithelial cell marker) was down-regulated in tumors of mice folJournal of oncology8777697https://www.ebi.ac.uk/biostudies/studies/S-EPMC9077451biostudies-literatureThe tRNA-Derived Fragment tRF-24-V29K9UV3IU Functions as a miRNA-like RNA to Prevent Gastric Cancer Progression by Inhibiting GPR78 Expression.PMC9077451Fan XYu SHuang WZhang YHe XWang HChen SfalseThe tRNA-Derived Fragment tRF-24-V29K9UV3IU Functions as a miRNA-like RNA to Prevent Gastric Cancer Progression by Inhibiting GPR78 Expression.Emerging studies have proved that tRNA-derived fragments (tRFs) play vital roles in tumor metastasis; however, the function of tRFs in gastric cancer (GC) remains largely unclear. We investigated the role of tRF-24-V29K9UV3IU in growth and metastasis of GC using a xenograft mouse model. Differential gene expression downstream of tRF-24-V29K9UV3IU was identified by transcriptome sequencing, and interaction was then verified by a dual luciferase reporter and RNA immunoprecipitation. MKN-45 cells were also used to explore the biological functions of tRF-24-V29K9UV3IU <i>in vitro</i>. Here, knockdown of tRF-24-V29K9UV3IU promoted tumor growth and metastasis of GC <i>in vivo</i>. The expression of tRF-24-V29K9UV3IU and E-cadherin (epithelial cell marker) was down-regulated in tumors of mice fol2022-01-01T00:00:00Z20222025-04-04T09:10:24.535Z2025-02-19T03:27:16.113ZS-EPMC90774513553530910.1155/2022/8777697