biostudies-literatureSpitzer TLNICHD NIH HHSeunice kennedy shriver national institute of child health and human development1262-1270https://www.ebi.ac.uk/biostudies/studies/S-EPMC9078052biostudies-literatureUnknown29(4)To study if stress, as measured by salivary alpha-amylase and cortisol, negatively impacts male fertility, as measured by semen parameters, pregnancy, and live birth rates. Prospective, cohort study of men enrolled in the Males, Antioxidants, and Infertility (MOXI) trial. One-hundred twelve infertile men provided first-morning salivary and semen samples at baseline. Salivary samples were analyzed for alpha-amylase and cortisol. Couples attempted to conceive naturally (months 1-3) and with clomiphene citrate/intrauterine insemination (months 4-6). The association between stress-related biomarkers and semen parameters including DNA fragmentation was assessed using linear regression models adjusting for male age. Salivary levels were dichotomized at the 80th percentile. Pregnancy/live birth rReproductive sciences (Thousand Oaks, Calif.)Biomarkers of Stress and Male Fertility.PMC9078052U10HD077844U10HD027049U10HD077680U10HD055925U10HD038992R25 HD075737U10 HD077680U10HD077841U10HD039005Santoro NZhang HSpitzer TLCoward RMKrawetz SASun FCedars MISteiner AZTrussell JCHansen KRBarnhart KTDiamond MPfalseBiomarkers of Stress and Male Fertility.To study if stress, as measured by salivary alpha-amylase and cortisol, negatively impacts male fertility, as measured by semen parameters, pregnancy, and live birth rates. Prospective, cohort study of men enrolled in the Males, Antioxidants, and Infertility (MOXI) trial. One-hundred twelve infertile men provided first-morning salivary and semen samples at baseline. Salivary samples were analyzed for alpha-amylase and cortisol. Couples attempted to conceive naturally (months 1-3) and with clomiphene citrate/intrauterine insemination (months 4-6). The association between stress-related biomarkers and semen parameters including DNA fragmentation was assessed using linear regression models adjusting for male age. Salivary levels were dichotomized at the 80th percentile. Pregnancy/live birth r2022-01-01T00:00:00Z2022 Apr2025-04-26T04:46:33.682Z2025-04-06T11:12:37.981ZS-EPMC90780523510674310.1007/s43032-022-00853-x