biostudies-literatureUnknown8(26)Li YThe immobilization of lipase on solid supports provides a significant improvement to the stability and reusability of lipase. During immobilization, the restricted surface area and inferior separation capacity of matrix materials are crucial for obtaining high-quality immobilized lipase. Carbon nitride nanosheets (C₃N₄-NS) as a type of two-dimensional nanomaterial have attracted various attentions for their prominent 2D planar nanostructure, characteristic surface area, thermostability and biocompatibility. Herein, we report a rational design and fabrication of immobilized <i>Candida rugosa</i> lipase based on carbon nitride nanosheets (C₃N₄-NS) as the matrix. The synthetic C₃N₄-NS are characterized by transmission electron RSC advances14229-14236https://www.ebi.ac.uk/biostudies/studies/S-EPMC9079863biostudies-literature<i>Candida rugosa</i> lipase covalently immobilized on facilely-synthesized carbon nitride nanosheets as a novel biocatalyst.PMC9079863Ruan ZHuang FLi YZheng MDeng QZheng CZhang SShi JTang Hfalse<i>Candida rugosa</i> lipase covalently immobilized on facilely-synthesized carbon nitride nanosheets as a novel biocatalyst.The immobilization of lipase on solid supports provides a significant improvement to the stability and reusability of lipase. During immobilization, the restricted surface area and inferior separation capacity of matrix materials are crucial for obtaining high-quality immobilized lipase. Carbon nitride nanosheets (C₃N₄-NS) as a type of two-dimensional nanomaterial have attracted various attentions for their prominent 2D planar nanostructure, characteristic surface area, thermostability and biocompatibility. Herein, we report a rational design and fabrication of immobilized <i>Candida rugosa</i> lipase based on carbon nitride nanosheets (C₃N₄-NS) as the matrix. The synthetic C₃N₄-NS are characterized by transmission electron 2018-01-01T00:00:00Z2018 Apr2025-04-19T09:20:53.364Z2025-04-19T09:20:53.364ZS-EPMC90798633554073910.1039/c8ra00536b