biostudies-literatureZhu TMajor Science and Technology Projects in Yunnan Province867525https://www.ebi.ac.uk/biostudies/studies/S-EPMC9081565biostudies-literatureUnknown13This study aimed to elucidate the mechanism of <i>Bletilla striata</i> oligosaccharides (BO) in the treatment of ulcerative colitis (UC). A UC mouse model was induced by 3% Dextran sodium sulfate (DSS), and BO (200 mg/kg/d) were administered for intervention. The results show that BO effectively inhibited the release of intestinal inflammatory cytokines such as IL-6, TNF-α, and IL-1β. Also, BO profoundly elevated the secretion of mucins and the expression of tight junction (TJ) proteins to attenuate dysfunction of the intestinal barrier. The 16S rDNA sequencing and liquid chromatography/gas chromatography-mass spectrometer (LC/GC-MS) analysis of mouse feces revealed that BO regulated the disturbance of gut microbiota and intestinal metabolites. By using the <i>in vitro</i> fermentation broFrontiers in pharmacology<i>Bletilla striata</i> Oligosaccharides Improve Ulcerative Colitis by Regulating Gut Microbiota and Intestinal Metabolites in Dextran Sulfate Sodium-Induced Mice.PMC9081565202102AE090042Liu HLiu YLi SZhang ZYe CHu HYin MHu BZhu Tfalse<i>Bletilla striata</i> Oligosaccharides Improve Ulcerative Colitis by Regulating Gut Microbiota and Intestinal Metabolites in Dextran Sulfate Sodium-Induced Mice.This study aimed to elucidate the mechanism of <i>Bletilla striata</i> oligosaccharides (BO) in the treatment of ulcerative colitis (UC). A UC mouse model was induced by 3% Dextran sodium sulfate (DSS), and BO (200 mg/kg/d) were administered for intervention. The results show that BO effectively inhibited the release of intestinal inflammatory cytokines such as IL-6, TNF-α, and IL-1β. Also, BO profoundly elevated the secretion of mucins and the expression of tight junction (TJ) proteins to attenuate dysfunction of the intestinal barrier. The 16S rDNA sequencing and liquid chromatography/gas chromatography-mass spectrometer (LC/GC-MS) analysis of mouse feces revealed that BO regulated the disturbance of gut microbiota and intestinal metabolites. By using the <i>in vitro</i> fermentation bro2022-01-01T00:00:00Z20222025-04-05T13:23:35.74Z2025-02-19T03:27:28.018ZS-EPMC90815653554833110.3389/fphar.2022.867525