{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yoshikawa H"],"funding":["Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering","Japan Society for the Promotion of Science"],"pagination":["14510-14514"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9082109"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(26)"],"pubmed_abstract":["We evaluated optical pickup ELISA with an original microfluidic disk that contains eight radially arranged channels, which enable semi-automatic sample loading and washing. This disk-shaped chip composed of acrylic plates was fabricated by CO<sub>2</sub> laser machining and capture antibodies were immobilized in the channels. After the immunoreaction with antigens and enzyme-linked secondary antibodies, an enzyme-catalyzed nanoaggregation of <i>o</i>-phenylenediamine was detected by measuring the reflectivity change of a laser beam focused in the channel. The assay of C-reactive protein (CRP) was successfully performed in a short amount of time (approximately 20 min from CRP loading). The limit of detection was determined to be 2 ng mL<sup>-1</sup>, which is more sensitive as compared with"],"journal":["RSC advances"],"pubmed_title":["An optical pickup enzyme-linked immunosorbent assay (ELISA) with a microfluidic disk."],"pmcid":["PMC9082109"],"funding_grant_id":["15H05769"],"pubmed_authors":["Yoshikawa H","Yoshinaga M","Tamiya E"],"additional_accession":[]},"is_claimable":false,"name":"An optical pickup enzyme-linked immunosorbent assay (ELISA) with a microfluidic disk.","description":"We evaluated optical pickup ELISA with an original microfluidic disk that contains eight radially arranged channels, which enable semi-automatic sample loading and washing. This disk-shaped chip composed of acrylic plates was fabricated by CO<sub>2</sub> laser machining and capture antibodies were immobilized in the channels. After the immunoreaction with antigens and enzyme-linked secondary antibodies, an enzyme-catalyzed nanoaggregation of <i>o</i>-phenylenediamine was detected by measuring the reflectivity change of a laser beam focused in the channel. The assay of C-reactive protein (CRP) was successfully performed in a short amount of time (approximately 20 min from CRP loading). The limit of detection was determined to be 2 ng mL<sup>-1</sup>, which is more sensitive as compared with","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Apr","modification":"2025-04-05T13:24:08.55Z","creation":"2025-04-05T13:24:08.55Z"},"accession":"S-EPMC9082109","cross_references":{"pubmed":["35540764"],"doi":["10.1039/c8ra01149d"]}}